大鼠模型正畸复发期间白细胞介素-6 和 microRNA-146A 的表达模式。

IF 2 4区 医学 Q3 PHYSIOLOGY Journal of Physiology and Pharmacology Pub Date : 2024-08-01 Epub Date: 2024-10-10 DOI:10.26402/jpp.2024.4.10
J-Y Dai, S-Q Li, X Jia, M-Y Wu, B Song, J-Y Li, Y Guo, R Gao
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引用次数: 0

摘要

在这项研究中,我们建立了一个模拟正畸牙齿移动复发的实验大鼠模型和一个人类牙周韧带干细胞(PDLScs)模型。我们的目的是探索牙周组织中 microRNA-146a (miR-146a)的表达、炎症因子白细胞介素-6(IL-6)和机械干预后正畸复发之间的关系。在动物实验中,30 只健康雄性 Wistar 大鼠被随机分配到对照组(n=6)或模型组(n=24)。模型组在施力 14 天后取下正畸矫治器。在移除正畸装置后的第 0、7、14 和 21 天收集牙龈缝隙液(GcF)和牙周组织样本,以评估 miR-146a 和 IL-6 表达的变化。在体外细胞培养研究中,在加入转染试剂 24 小时后分离人类前磨牙组织,以收获 PDLScs。反转录定量聚合酶链反应用于评估 miRNA-146a 基因的表达水平,而 Western 印迹分析则用于评估 IL-6 蛋白的生成。结果显示,与对照组相比,IL-6 蛋白表达量在模型第 7 天组明显升高至峰值(p0.05)。
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Expression patterns of interleukin-6 and microRNA-146A during orthodontic relapse in a rat model.

In this study, we established an experimental rat model to simulate orthodontic tooth movement relapse and a human periodontal ligament stem cell (PDLScs) model. Our aim was to explore the relationship between microRNA-146a (miR-146a) expression in periodontal tissue, the inflammatory factor interleukin-6 (IL-6), and orthodontic relapse subsequent to mechanical intervention. In the animal experiment, a total of 30 healthy male Wistar rats were randomly allocated to either the control group (n=6) or the model group (n=24). In the model group, the orthodontic appliance was removed 14 days after force application. Gingival crevicular fluid (GcF) and periodontal tissue samples were collected at intervals of days 0, 7, 14, and 21 following removal of the orthodontic appliance to assess alterations in miR-146a and IL-6 expressions. In the in vitro cell culture study, human premolar tooth tissue was isolated 24 hours following the addition of the transfection reagent to harvest PDLScs. Reverse transcription quantitative polymerase chain reaction was employed to evaluate the expression levels of the miRNA-146a gene, while Western blot analysis was utilized to assess the production of the IL-6 protein. As a result in comparison to the control group, the protein expression of IL-6 notably escalated to its peak value in the model-day 7 group (p<0.05). Subsequently, although experiencing a slight decline, the IL-6 expression in the model-day 14 group remained significantly elevated compared to control group (p<0.05). In the model-day 21 group, the protein expression of IL-6 approached that of the control group, with no significant difference observed (p>0.05). conversely, in relation to the control group, the gene expression of miR-146a drastically decreased to its lowest point in the model-day 7 group (p<0.05). While exhibiting a slight increase, the miR-146a expression in the model-day 14 group remained significantly diminished compared to control group (p<0.05). Following the identification of human periodontal ligament cells (hPDLcs) through immunofluorescence in the in vitro study, a subsequent experiment was conducted to specifically inhibit miR-146a expression. In comparison to the control group, the protein expression of IL-6 demonstrated a significant increase in the anti-miRNA oligodeoxyribonucleotide (AMO) group, where miR-146a expression was effectively suppressed (p<0.05). Throughout the process of orthodontic tooth movement relapse in rats, there was a notable reduction in the gene expression of miR-146a, accompanied by a significant increase in the expression of IL-6.

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来源期刊
CiteScore
4.00
自引率
22.70%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Journal of Physiology and Pharmacology publishes papers which fall within the range of basic and applied physiology, pathophysiology and pharmacology. The papers should illustrate new physiological or pharmacological mechanisms at the level of the cell membrane, single cells, tissues or organs. Clinical studies, that are of fundamental importance and have a direct bearing on the pathophysiology will also be considered. Letters related to articles published in The Journal with topics of general professional interest are welcome.
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