白蚁RPL23基因在白蚁抗Metarhizium anisopliae主动免疫中的作用研究

IF 3.6 3区 生物学 Q1 ZOOLOGY Journal of invertebrate pathology Pub Date : 2024-10-22 DOI:10.1016/j.jip.2024.108226
Shuxin Yu , Ali Hassan , Nasir Mehmood , Wei Zhou , Taqi Raza , Qiuying Huang
{"title":"白蚁RPL23基因在白蚁抗Metarhizium anisopliae主动免疫中的作用研究","authors":"Shuxin Yu ,&nbsp;Ali Hassan ,&nbsp;Nasir Mehmood ,&nbsp;Wei Zhou ,&nbsp;Taqi Raza ,&nbsp;Qiuying Huang","doi":"10.1016/j.jip.2024.108226","DOIUrl":null,"url":null,"abstract":"<div><div>Ribosomal proteins are considered to be involved in the immunity of different animals against pathogens. The protein level of RPL23 increased after fungal infection in termites, but how it influence active immunity in termites is unknown. The role of <em>RPL23</em> gene was studied to evaluate its impact on active immunity of termite <em>Reticulitermes chinensis</em> against entomopathogenic fungus (EPF) <em>Metarhizium anisopliae</em>. The <em>RPL23</em> gene fragment (414 bp) was cloned and phylogenetic analysis revealed that it’s very close to termite <em>Coptotermes formosanus</em>. Expression of <em>RPL23</em> gene was significantly higher in abdomen as compared to thorax and head. Silencing <em>RPL23</em> gene had no significant impact on the frequency and time of allogrooming towards fungus exposed termites from nestmates, which showed that nestmates acquired spores from infected termites through allogrooming. Expression of immune genes (<em>GNBP1</em>, <em>GNBP2</em> and phenoloxidase) and apoptosis related genes (<em>TNF-α,</em> caspase 1, caspase 3 and caspase 8) decreased significantly in nestmates of fungus-treated termites after silencing of <em>RPL23</em> gene as compared to control. Antifungal activity and survival of <em>RPL23</em> silenced nestmates of fungus-treated termites also decreased. To sum up, this study found that silencing of <em>RPL23</em> gene broke the active immunity against <em>M. anisopliae</em> infection, reduced the antifungal activity of termites, weakened cell apoptosis, and led to increased mortality of termites, which may help to find a potential alternative for chemical insecticides to control termites.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108226"},"PeriodicalIF":3.6000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study on the role of RPL23 gene in active immunity of termite Reticulitermes chinensis against Metarhizium anisopliae\",\"authors\":\"Shuxin Yu ,&nbsp;Ali Hassan ,&nbsp;Nasir Mehmood ,&nbsp;Wei Zhou ,&nbsp;Taqi Raza ,&nbsp;Qiuying Huang\",\"doi\":\"10.1016/j.jip.2024.108226\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Ribosomal proteins are considered to be involved in the immunity of different animals against pathogens. The protein level of RPL23 increased after fungal infection in termites, but how it influence active immunity in termites is unknown. The role of <em>RPL23</em> gene was studied to evaluate its impact on active immunity of termite <em>Reticulitermes chinensis</em> against entomopathogenic fungus (EPF) <em>Metarhizium anisopliae</em>. The <em>RPL23</em> gene fragment (414 bp) was cloned and phylogenetic analysis revealed that it’s very close to termite <em>Coptotermes formosanus</em>. Expression of <em>RPL23</em> gene was significantly higher in abdomen as compared to thorax and head. Silencing <em>RPL23</em> gene had no significant impact on the frequency and time of allogrooming towards fungus exposed termites from nestmates, which showed that nestmates acquired spores from infected termites through allogrooming. Expression of immune genes (<em>GNBP1</em>, <em>GNBP2</em> and phenoloxidase) and apoptosis related genes (<em>TNF-α,</em> caspase 1, caspase 3 and caspase 8) decreased significantly in nestmates of fungus-treated termites after silencing of <em>RPL23</em> gene as compared to control. Antifungal activity and survival of <em>RPL23</em> silenced nestmates of fungus-treated termites also decreased. To sum up, this study found that silencing of <em>RPL23</em> gene broke the active immunity against <em>M. anisopliae</em> infection, reduced the antifungal activity of termites, weakened cell apoptosis, and led to increased mortality of termites, which may help to find a potential alternative for chemical insecticides to control termites.</div></div>\",\"PeriodicalId\":16296,\"journal\":{\"name\":\"Journal of invertebrate pathology\",\"volume\":\"207 \",\"pages\":\"Article 108226\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-10-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of invertebrate pathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022201124001691\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201124001691","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

核糖体蛋白被认为参与了不同动物对病原体的免疫。白蚁感染真菌后,RPL23 蛋白水平升高,但它如何影响白蚁的主动免疫尚不清楚。本研究对 RPL23 基因的作用进行了研究,以评估其对白蚁抵抗昆虫病原真菌 Metarhizium anisopliae 的主动免疫能力的影响。克隆了 RPL23 基因片段(414 bp),系统进化分析表明它与白蚁 Coptotermes formosanus 非常接近。RPL23基因在腹部的表达量明显高于胸部和头部。沉默RPL23基因对白蚁从巢友那里获取暴露于真菌的孢子的频率和时间没有明显影响,这表明巢友是通过异交从受感染的白蚁那里获取孢子的。与对照组相比,RPL23基因沉默后,真菌处理白蚁巢内免疫基因(GNBP1、GNBP2和酚氧化酶)和细胞凋亡相关基因(TNF-α、caspase 1、caspase 3和caspase 8)的表达量明显下降。RPL23基因沉默后,真菌处理的白蚁巢员的抗真菌活性和存活率也有所下降。综上所述,本研究发现,RPL23基因沉默打破了白蚁对M. anisopliae感染的主动免疫,降低了白蚁的抗真菌活性,削弱了细胞凋亡,导致白蚁死亡率增加,这可能有助于找到一种潜在的化学杀虫剂替代品来控制白蚁。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Study on the role of RPL23 gene in active immunity of termite Reticulitermes chinensis against Metarhizium anisopliae
Ribosomal proteins are considered to be involved in the immunity of different animals against pathogens. The protein level of RPL23 increased after fungal infection in termites, but how it influence active immunity in termites is unknown. The role of RPL23 gene was studied to evaluate its impact on active immunity of termite Reticulitermes chinensis against entomopathogenic fungus (EPF) Metarhizium anisopliae. The RPL23 gene fragment (414 bp) was cloned and phylogenetic analysis revealed that it’s very close to termite Coptotermes formosanus. Expression of RPL23 gene was significantly higher in abdomen as compared to thorax and head. Silencing RPL23 gene had no significant impact on the frequency and time of allogrooming towards fungus exposed termites from nestmates, which showed that nestmates acquired spores from infected termites through allogrooming. Expression of immune genes (GNBP1, GNBP2 and phenoloxidase) and apoptosis related genes (TNF-α, caspase 1, caspase 3 and caspase 8) decreased significantly in nestmates of fungus-treated termites after silencing of RPL23 gene as compared to control. Antifungal activity and survival of RPL23 silenced nestmates of fungus-treated termites also decreased. To sum up, this study found that silencing of RPL23 gene broke the active immunity against M. anisopliae infection, reduced the antifungal activity of termites, weakened cell apoptosis, and led to increased mortality of termites, which may help to find a potential alternative for chemical insecticides to control termites.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
期刊最新文献
Bombyx mori PAT4 gene inhibits BmNPV infection and replication through autophagy Inheritance of Cry2Ab2 resistance in two Helicoverpa zea (Lepidoptera: Noctuidae) populations resistant to single- and dual-Bacillus thuringiensis proteins A histopathological guide for the social spider Stegodyphus dumicola Identification of Albopleistophora grylli n. gen. n. sp. (Microsporidia) and its impact on crickets (Gryllus spp.) in food-and-feed culture systems Distribution and genetic diversity of Bombyx mori nucleopolyhedrovirus in mass-reared silkworms in Thailand
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1