结合微生物组学和代谢组学分析蜂蜜加工黄芪多糖改善脾气虚小鼠肠道免疫功能的证据

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2024-10-27 DOI:10.1002/jsfa.13986

Meng Jia, Meng Yuan, Xuqi Zhu, Danna Lin, Xueying Li, Li Huang, Hongyuan Chen, Wen Rui

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引用次数: 0

摘要

背景介绍黄芪（俗称黄芪）是一种传统的药用和食用植物，具有补气的功效。与生品相比，用蜂蜜加工的黄芪剂型（即蜂蜜加工黄芪（HPA））显示出更好的补气功效。多糖是其水提取物中的主要生物活性成分。本研究采用微生物组学和代谢组学相结合的多组学方法，阐明了蜂蜜加工黄芪多糖（HAPS）的补气机制：结果：与脾气虚小鼠相比，HAPS治疗小鼠的症状评分、脾脏和胸腺指数、血清细胞因子（肿瘤坏死因子α、白细胞介素1β）和肠黏膜分泌免疫球蛋白A（SIgA）均有所改善。肠道微生物群分析表明，HAPS能调节类杆菌、乳酸杆菌、变形杆菌和螺旋杆菌的相对丰度，从而改善脾气虚小鼠的肠道菌群失调。在粪便代谢组学分析中筛选并确定了11个生物标志物，主要与亚油酸代谢、鞘脂代谢、甘油磷脂代谢和不饱和脂肪酸的生物合成有关。此外，综合分析表明，HAPS通过调节类杆菌的丰度来调节棕榈酸和鞘脂的代谢，进而提高脾气虚小鼠肠粘膜SIgA的水平，恢复肠粘膜免疫功能：我们的研究结果表明，HAPS 是 HPA 的重要活性成分，其补气机制与肠道免疫功能的改善密切相关。这些发现为 HAPS 作为免疫调节剂应用于保健和膳食食品奠定了基础。© 2024 化学工业协会。

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Evidence of honey-processed Astragalus polysaccharides improving intestinal immune function in spleen Qi deficiency mice integrated with microbiomics and metabolomics analysis.

Background: Radix Astragali, commonly known as Astragalus, is a traditional medicinal and edible plant valued for its Qi-tonifying properties. The dosage form of Radix Astragali processed with honey, known as honey-processed Astragalus (HPA), shows improved Qi-tonifying efficacy as compared to the raw product. Polysaccharides are the main bioactive ingredients in its aqueous extract. This study used a multiomics approach integrating microbiomics and metabolomics to elucidate the Qi-tonifying mechanisms of honey-processed Astragalus polysaccharides (HAPS).

Results: HAPS-treated mice showed improved symptom scores, spleen and thymus indices, serum cytokines (tumor necrosis factor α, interleukin 1β) and intestinal mucosa secretory immunoglobulin A (SIgA) compared to the mice with spleen Qi deficiency. The analysis of gut microbiota indicated that HAPS regulated the relative abundance of Bacteroidetes, Bacteroides, Proteobacteria and Helicobacter, thereby improving intestinal flora dysbiosis in mice with spleen Qi deficiency. Eleven biomarkers in fecal metabolomics analysis were screened and identified, primarily associated with linoleic acid metabolism, sphingolipid metabolism, glycerophospholipid metabolism and biosynthesis of unsaturated fatty acids. Furthermore, comprehensive analyses demonstrated that HAPS regulates palmitic acid and sphingolipid metabolism by modulating the abundance of Bacteroidetes, which in turn increased the levels of intestinal mucosal SIgA and restored intestinal mucosal immune function in mice with spleen Qi deficiency.

Conclusion: Our findings revealed that HAPS is an essential active ingredient of HPA, and its Qi-tonifying mechanism is closely related to the improvement of intestinal immune function. These findings lay the foundation for the application of HAPS as an immunomodulatory agent in health and dietary foods. © 2024 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.