梁平柚（Citrus maxima cv. Liangpin Yu）果皮中高甲氧基 HG 型果胶的制备、鉴定和 α-葡萄糖苷酶抑制活性。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2024-10-23 DOI:10.1002/jsfa.13967

Xin Gu, Tao Gao, Mengya Liu, Yakun Hou, Zhonggui Jiang, Yuhao Zhang

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引用次数: 0

摘要

背景：梁平柚（Citrus maxima cv. Liangpin Yu）的果皮在采后处理和加工过程中通常被作为废物丢弃，造成环境污染和浪费。果胶是柚子皮中的主要成分，具有显著的经济效益。因此，开发柚子皮果胶（PPs）可能是减少柚子皮环境污染的可行策略：结果：在接种量为 100 mg g-1、液固比为 31 mL g-1、发酵时间为 64 h、发酵温度为 39 ℃的条件下，PPs 的最佳产量为 156.5 ± 2.5 g kg-1。经 DEAE-52 纤维素、Sephadex G-100 和 Sephadex G-75 柱层析纯化的 PPs 中的果胶组分 PPs-6Aa 显示出较高的α-葡萄糖苷酶抑制活性，其 IC50 为 0.12 ± 0.03 mg mL-1。它是一种 42.8 kDa 的高甲氧基 HG 型果胶，其重复单元为 →4)-α-GalpA-6-OMe-(1→4)-α-GalpA-6-OMe-(1→)。此外，其α-葡萄糖苷酶抑制活性可能与 Lys-156、Glu-277、His-280、Asp-307、Arg-315、Asn-350、Asp-352 和 Glu-411 形成的氢键，以及与 Ser-157、Tyr-158、Asp-233、Gln-239、Ser-240、Phe-303、Thr-306、Leu-313、Phe-314、Gln-353 和 Arg-442 形成的疏水相互作用有关：这些发现提供了梁平柚皮果胶的结构和生物活性信息，有利于功能食品和药物的开发。© 2024 化学工业协会。

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Preparation, identification and α-glucosidase inhibitory activity of a high-methoxyl HG-type pectin from Liangping pomelo (Citrus maxima cv. Liangpin Yu) peel.

Background: The peel from Liangping pomelo (Citrus maxima cv. Liangpin Yu) is generally discarded as waste during post-harvest handling and process, resulting in environmental pollution and waste. Pectin is the major component in pomelo peels and yields significant economic advantages. Thus, developing pomelo peel pectin (PPs) might be a feasible strategy to reduce environmental pollution caused by pomelo peel.

Results: The optimized PPs yield was 156.5 ± 2.5 g kg^-1 under the inoculum size of 100 mg g^-1, liquid-solid ratio of 31 mL g^-1, fermentation time of 64 h, and fermentation temperature of 39 °C. PPs-6Aa, a pectin fraction from PPs purified with DEAE-52 cellulose, Sephadex G-100 and Sephadex G-75 column chromatography, showed higher α-glucosidase inhibitory activity, with an IC₅₀ of 0.12 ± 0.03 mg mL^-1. It was a high-methoxyl HG-type pectin of 42.8 kDa, and its repeat unit was →4)-α-GalpA-6-OMe-(1→4)-α-GalpA-6-OMe-(1→. Additionally, its α-glucosidase inhibitory activity might be related to hydrogen bonds formed with Lys-156, Glu-277, His-280, Asp-307, Arg-315, Asn-350, Asp-352 and Glu-411, and to hydrophobic interactions formed with Ser-157, Tyr-158, Asp-233, Gln-239, Ser-240, Phe-303, Thr-306, Leu-313, Phe-314, Gln-353 and Arg-442.

Conclusion: These findings provide structural and bioactivity information on pectin from Liangping pomelo peel, which could be beneficial for the development of functional foods and pharmaceuticals. © 2024 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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