探索不同脂肪细胞模型的形态和分子特性:单层、球形、结冷胶水凝胶和外植体

IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Macromolecular bioscience Pub Date : 2024-10-25 DOI:10.1002/mabi.202400320
Franziska B Albrecht, Ann-Kathrin Schick, Annemarie Klatt, Freia F Schmidt, Svenja Nellinger, Petra J Kluger
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引用次数: 0

摘要

白色脂肪组织(WAT)在能量平衡中起着至关重要的作用,并能分泌多种具有深远影响的脂肪因子。白脂肪组织与糖尿病、心血管疾病和癌症等疾病有关。研究疾病和组织代谢需要大量合适的体外模型。这些模型大多采用二维单层培养。本研究旨在评估不同 WAT 模型的性能,以更好地推导其潜在应用。研究分析了球形和两种三维结冷胶水凝胶与原位小叶和二维单层培养中脂肪细胞特性的稳定性。首先,确定分化以获得类似脂肪细胞的特征。其次,为了评估已分化的基于 ASC 的模型、基于脂肪细胞的模型和外植体在 3 周内的维持情况,对活力、细胞内脂质含量、过脂素 A 表达、脂肪因子和基因表达进行了分析。每种模型都有若干优点。包括但不限于二维单层中的强分化、球体内的自组装、含干细胞水凝胶的长期稳定性以及含脂肪细胞水凝胶和小叶内的成熟表型。这项研究强调了三维模型的优势,因为它们更像活体行为,并概述了不同的脂肪细胞模型。
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Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants.

White adipose tissue (WAT) plays a crucial role in energy homeostasis and secretes numerous adipokines with far-reaching effects. WAT is linked to diseases such as diabetes, cardiovascular disease, and cancer. There is a high demand for suitable in vitro models to study diseases and tissue metabolism. Most of these models are covered by 2D-monolayer cultures. This study aims to evaluate the performance of different WAT models to better derive potential applications. The stability of adipocyte characteristics in spheroids and two 3D gellan gum hydrogels with ex situ lobules and 2D-monolayer culture is analyzed. First, the differentiation to achieve adipocyte-like characteristics is determined. Second, to evaluate the maintenance of differentiated ASC-based models, an adipocyte-based model, and explants over 3 weeks, viability, intracellular lipid content, perilipin A expression, adipokine, and gene expression are analyzed. Several advantages are supported using each of the models. Including, but not limited to, the strong differentiation in 2D-monolayers, the self-assembling within spheroids, the long-term stability of the stem cell-containing hydrogels, and the mature phenotype within adipocyte-containing hydrogels and the lobules. This study highlights the advantages of 3D models due to their more in vivo-like behavior and provides an overview of the different adipose cell models.

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来源期刊
Macromolecular bioscience
Macromolecular bioscience 生物-材料科学:生物材料
CiteScore
7.90
自引率
2.20%
发文量
211
审稿时长
1.5 months
期刊介绍: Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.
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