Mikela Vlachou, Andreana Pexara, Nikolaos Solomakos, Alexander Govaris, Dimitrios Palaiogiannis, Vassilis Athanasiadis, Stavros I Lalas
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引用次数: 0
摘要
这项研究旨在评估希腊屠宰猪血清中的赭曲霉毒素A（OTA）残留量。样本来自希腊8个不同地区21个区域单位的113个不同农场的1695头健康屠宰猪，并采用免疫吸附分析法（ELISA）和高效液相色谱-荧光检测器（HPLC-FD）进行分析。OTA 污染评估显示，分别有 782 份（46.1%）和 1233 份（72.7%）样本 OTA 阳性，根据 ELISA 和 HPLC-FD 分析，浓度范围分别为 0.20-5.38 微克/升和 0.15-5.96 微克/升。此外，通过 ELISA 和 HPLC-FD 分析，分别发现 88 个（77.9%）和 108 个（95.6%）猪场的 OTA 呈阳性。Thessaly 地区的 OTA 血清阳性率（>98%）和 HPLC-FD 测定的毒素水平（5.96 µg/L）最高，而克里特岛地区猪场的 OTA 阳性率高达 100%（范围 75-100%）。在希腊不同地区的屠宰猪血清中检测到 OTA 会对动物和人类健康造成威胁，因此需要对养猪业和猪肉生产设施进行持续的 OTA 监测。
Presence of Ochratoxin A Residues in Blood Serum of Slaughtered Pigs in Greece.
This study aimed to assess the presence of ochratoxin A (OTA) residues in the blood serum of slaughtered pigs in Greece. Samples were obtained from 1695 healthy slaughtered pigs originating from 113 different farms located in 21 geographic regional units in 8 different geographic regions of Greece and were analyzed using an immunosorbent assay (ELISA) and high-performance liquid chromatography with fluorescence detector (HPLC-FD). OTA contamination assessment showed that 782 (46.1%) and 1233 (72.7%) samples were OTA-positive, with a concentration range of 0.20-5.38 μg/L and 0.15-5.96 µg/L according to ELISA and HPLC-FD analysis, respectively. Also, 88 (77.9%) and 108 (95.6%) of farms were found to be OTA-positive by ELISA and HPLC-FD analysis, respectively. The highest OTA serum positivity rate (>98%) and toxin level (5.96 µg/L) determined by HPLC-FD were observed in the Thessaly region, whereas a high prevalence of up to 100% (range 75-100%) was found on farms in the Crete Island region. The detection of OTA in the serum of slaughtered pigs in different regions in Greece poses a risk for animal and human health and highlights the need for constant OTA monitoring in the swine industry and pork meat production facilities.
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