Nikita David, Emma Ivantsova, Isaac Konig, Cole D English, Lev Avidan, Mark Kreychman, Mario L Rivera, Camilo Escobar, Eliana Maira Agostini Valle, Amany Sultan, Christopher J Martyniuk
{"title":"幼年斑马鱼(Danio rerio)暴露于全氟辛烷磺酰胺(PFOSA)后的不良后果:从神经毒性和行为角度看全氟辛烷磺酰胺。","authors":"Nikita David, Emma Ivantsova, Isaac Konig, Cole D English, Lev Avidan, Mark Kreychman, Mario L Rivera, Camilo Escobar, Eliana Maira Agostini Valle, Amany Sultan, Christopher J Martyniuk","doi":"10.3390/toxics12100723","DOIUrl":null,"url":null,"abstract":"<p><p>Toxicity mechanisms of per- and polyfluoroalkyl substances (PFASs), a chemical class present in diverse ecosystems, as well as many of their precursors, have been increasingly characterized in aquatic species. Perfluorooctanesulfonamide (PFOSA, C<sub>8</sub>H<sub>2</sub>F<sub>17</sub>NO<sub>2</sub>S) is a common precursor of perfluorooctane sulfonic acid (PFOS), a long-chain PFAS. Here, we assessed sub-lethal endpoints related to development, oxidative stress, transcript levels, and distance moved in zebrafish embryos and larvae following continuous exposure to PFOSA beginning at 6 h post-fertilization (hpf). PFOSA decreased survival in fish treated with 1 µg/L PFOSA; however, the effect was modest relative to the controls (difference of 10%). Exposure up to 10 µg/L PFOSA did not affect hatch rate, nor did it induce ROS in 7-day-old larvae fish. The activity of larval fish treated with 100 µg/L PFOSA was reduced relative to the solvent control. Transcripts related to oxidative stress response and apoptosis were measured and BCL2-associated X, apoptosis regulator (<i>bax</i>)<i>,</i> cytochrome c, somatic (<i>cycs</i>)<i>,</i> catalase (<i>cat</i>), superoxide dismutase 2 (<i>sod2</i>) were induced with high concentrations of PFOSA. Genes related to neurotoxicity were also measured and transcript levels of acetylcholinesterase (<i>ache</i>), elav-like RNA binding protein 3 (<i>elavl3</i>), growth-associated protein 43 (<i>gap43</i>), synapsin II (<i>syn2a</i>), and tubulin <i>3 (tubb3)</i> were all increased in larval fish with higher PFOSA exposure. These data improve our understanding of the potential sub-lethal toxicity of PFOSA in fish species.</p>","PeriodicalId":23195,"journal":{"name":"Toxics","volume":"12 10","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510739/pdf/","citationCount":"0","resultStr":"{\"title\":\"Adverse Outcomes Following Exposure to Perfluorooctanesulfonamide (PFOSA) in Larval Zebrafish (<i>Danio rerio</i>): A Neurotoxic and Behavioral Perspective.\",\"authors\":\"Nikita David, Emma Ivantsova, Isaac Konig, Cole D English, Lev Avidan, Mark Kreychman, Mario L Rivera, Camilo Escobar, Eliana Maira Agostini Valle, Amany Sultan, Christopher J Martyniuk\",\"doi\":\"10.3390/toxics12100723\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Toxicity mechanisms of per- and polyfluoroalkyl substances (PFASs), a chemical class present in diverse ecosystems, as well as many of their precursors, have been increasingly characterized in aquatic species. Perfluorooctanesulfonamide (PFOSA, C<sub>8</sub>H<sub>2</sub>F<sub>17</sub>NO<sub>2</sub>S) is a common precursor of perfluorooctane sulfonic acid (PFOS), a long-chain PFAS. Here, we assessed sub-lethal endpoints related to development, oxidative stress, transcript levels, and distance moved in zebrafish embryos and larvae following continuous exposure to PFOSA beginning at 6 h post-fertilization (hpf). PFOSA decreased survival in fish treated with 1 µg/L PFOSA; however, the effect was modest relative to the controls (difference of 10%). Exposure up to 10 µg/L PFOSA did not affect hatch rate, nor did it induce ROS in 7-day-old larvae fish. The activity of larval fish treated with 100 µg/L PFOSA was reduced relative to the solvent control. Transcripts related to oxidative stress response and apoptosis were measured and BCL2-associated X, apoptosis regulator (<i>bax</i>)<i>,</i> cytochrome c, somatic (<i>cycs</i>)<i>,</i> catalase (<i>cat</i>), superoxide dismutase 2 (<i>sod2</i>) were induced with high concentrations of PFOSA. Genes related to neurotoxicity were also measured and transcript levels of acetylcholinesterase (<i>ache</i>), elav-like RNA binding protein 3 (<i>elavl3</i>), growth-associated protein 43 (<i>gap43</i>), synapsin II (<i>syn2a</i>), and tubulin <i>3 (tubb3)</i> were all increased in larval fish with higher PFOSA exposure. 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Adverse Outcomes Following Exposure to Perfluorooctanesulfonamide (PFOSA) in Larval Zebrafish (Danio rerio): A Neurotoxic and Behavioral Perspective.
Toxicity mechanisms of per- and polyfluoroalkyl substances (PFASs), a chemical class present in diverse ecosystems, as well as many of their precursors, have been increasingly characterized in aquatic species. Perfluorooctanesulfonamide (PFOSA, C8H2F17NO2S) is a common precursor of perfluorooctane sulfonic acid (PFOS), a long-chain PFAS. Here, we assessed sub-lethal endpoints related to development, oxidative stress, transcript levels, and distance moved in zebrafish embryos and larvae following continuous exposure to PFOSA beginning at 6 h post-fertilization (hpf). PFOSA decreased survival in fish treated with 1 µg/L PFOSA; however, the effect was modest relative to the controls (difference of 10%). Exposure up to 10 µg/L PFOSA did not affect hatch rate, nor did it induce ROS in 7-day-old larvae fish. The activity of larval fish treated with 100 µg/L PFOSA was reduced relative to the solvent control. Transcripts related to oxidative stress response and apoptosis were measured and BCL2-associated X, apoptosis regulator (bax), cytochrome c, somatic (cycs), catalase (cat), superoxide dismutase 2 (sod2) were induced with high concentrations of PFOSA. Genes related to neurotoxicity were also measured and transcript levels of acetylcholinesterase (ache), elav-like RNA binding protein 3 (elavl3), growth-associated protein 43 (gap43), synapsin II (syn2a), and tubulin 3 (tubb3) were all increased in larval fish with higher PFOSA exposure. These data improve our understanding of the potential sub-lethal toxicity of PFOSA in fish species.
ToxicsChemical Engineering-Chemical Health and Safety
CiteScore
4.50
自引率
10.90%
发文量
681
审稿时长
6 weeks
期刊介绍:
Toxics (ISSN 2305-6304) is an international, peer-reviewed, open access journal which provides an advanced forum for studies related to all aspects of toxic chemicals and materials. It publishes reviews, regular research papers, and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in detail. There is, therefore, no restriction on the maximum length of the papers, although authors should write their papers in a clear and concise way. The full experimental details must be provided so that the results can be reproduced. Electronic files or software regarding the full details of calculations and experimental procedure can be deposited as supplementary material, if it is not possible to publish them along with the text.