多重 PCR 检测组(BioFire Joint Infection®)在调整经验性抗菌治疗中的临床实用性:小儿骨关节感染的经验。

IF 2 4区 医学 Q2 PEDIATRICS Children-Basel Pub Date : 2024-10-14 DOI:10.3390/children11101236
Clara Udaondo, Rosa María Alcobendas Rueda, Blanca Diaz-Delgado, Agustin Remesal, Inmaculada Quiles-Melero, Cristina Calvo
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引用次数: 0

摘要

背景/目的:本研究旨在评估 PCR 多反应面板(BioFire JI®)对小儿骨关节感染诊断和管理的影响:这项回顾性研究分析了 2023 年 1 月至 2024 年 4 月期间诊断为骨关节感染的儿科患者的数据。结果:共有 50 名患者被确诊为骨关节感染(其中 1 人死亡,1 人重症监护):共发现 50 名患者(66.6% 为男性,74% 年龄在 3 岁以下)。诊断结果如下:化脓性关节炎占 46%,骨髓炎占 26%,化脓性骨关节炎占 22%。有 22 例(44%)通过常规培养分离出了可确定的病原体。王氏菌是主要的病原体,占病例数的 50%(11/22),其次是金黄色葡萄球菌(9/22)。BioFire JI® Panel PCR 的灵敏度为 93%,与滑液培养作为参考标准相比,特异性为 63%。该小组还鉴定出了 7 种传统培养方法未检测到的病原体:2/9 种 MSSA(22%)、1/1 种 S.pyogenes(100%)和 4/11 种 K.kingae(37%),使鉴定率提高了 14%。病原体的快速鉴定有助于及时采取有针对性的治疗措施:结论:PCR多重检测板(BioFire JI®)提高了小儿骨关节感染的诊断率。
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Clinical Utility of a Multiplex PCR Panel (BioFire Joint Infection®) in the Adjustment of Empiric Antimicrobial Therapy: Experience in Pediatric Osteoarticular Infections.

Background/objectives: This study aims to evaluate the impact of the PCR multiplex panel (BioFire JI®) on the diagnosis and management of pediatric osteoarticular infections.

Methods: This retrospective study analyzed data from pediatric patients diagnosed with osteoarticular infections between January 2023 and April 2024. The effectiveness of the PCR multiplex panel in identifying pathogens was compared with traditional culture methods.

Results: In total, 50 patients were identified (66.6% male, 74% under 3 years of age). They were diagnosed as follows: septic arthritis in 46%, osteomyelitis in 26%, and septic osteoarthritis in 22%. An identifiable agent was isolated by conventional culture in 22 cases (44%). Kingella kingae was the predominant pathogen identified, accounting for 50% of cases (11/22), followed by Staphylococcus aureus (9/22). The BioFire JI® Panel PCR demonstrated a sensitivity of 93%, with a specificity of 63% when evaluated against synovial fluid culture as the reference standard. The panel identified seven additional pathogens not detected by conventional culture methods: 2/9 MSSA (22%), 1/1 S. pyogenes (100%), and 4/11 K. kingae (37%), increasing the yield by 14%. The rapid identification of pathogens facilitated timely and targeted therapeutic interventions.

Conclusions: The PCR multiplex panel (BioFire JI®) improved the diagnosis of pediatric osteoarticular infections.

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来源期刊
Children-Basel
Children-Basel PEDIATRICS-
CiteScore
2.70
自引率
16.70%
发文量
1735
审稿时长
6 weeks
期刊介绍: Children is an international, open access journal dedicated to a streamlined, yet scientifically rigorous, dissemination of peer-reviewed science related to childhood health and disease in developed and developing countries. The publication focuses on sharing clinical, epidemiological and translational science relevant to children’s health. Moreover, the primary goals of the publication are to highlight under‑represented pediatric disciplines, to emphasize interdisciplinary research and to disseminate advances in knowledge in global child health. In addition to original research, the journal publishes expert editorials and commentaries, clinical case reports, and insightful communications reflecting the latest developments in pediatric medicine. By publishing meritorious articles as soon as the editorial review process is completed, rather than at predefined intervals, Children also permits rapid open access sharing of new information, allowing us to reach the broadest audience in the most expedient fashion.
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