用于内皮细胞检测的非侵入性开关荧光生物传感器

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-10-09 DOI:10.3390/bios14100489
Qingyun Jiang, Shuai Shao, Na Li, Zhengyao Zhang, Bo Liu
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引用次数: 0

摘要

为了在不进行免疫染色的情况下快速方便地检测活体内皮细胞(EC),我们开发了一种基于开启荧光蛋白的生物传感器,命名为 LV-EcpG。它包括一个通过噬菌体展示技术获得的用于特异性识别 EC 的高亲和力肽 E12P 和一个融合了两个连接肽的开启型 EGFP。这种基因编码的基于荧光蛋白的生物传感器(FPB)的 "开关 "机制确保了只有在与心肌细胞结合时才会激活荧光信号,从而使这些 FPB 字符能够直接、可视和非侵入性地检测心肌细胞。它的特异性和多色成像能力使 LV-EcpG 成为活体心血管细胞研究的有力工具,在诊断和治疗心血管疾病及肿瘤血管生成方面具有巨大潜力。
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Non-Invasive On-Off Fluorescent Biosensor for Endothelial Cell Detection.

For rapid and convenient detection of living endothelial cells (ECs) specifically without immunostaining, we developed a biosensor based on turn-on fluorescent protein, named LV-EcpG. It includes a high-affinity peptide E12P obtained through phage display technology for specifically recognizing ECs and a turn-on EGFP fused with two linker peptides. The "on-off" switching mechanism of this genetically encoded fluorescent protein-based biosensor (FPB) ensured that fluorescence signals were activated only when binding with ECs, thus enabling these FPB characters for direct, visual, and non-invasive detection of ECs. Its specificity and multicolor imaging capability established LV-EcpG as a powerful tool for live EC research, with significant potential for diagnosing and treating cardiovascular diseases and tumor angiogenesis.

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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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