从双壳贝肉中提取麻痹性贝类毒素,利用电位化学传感器进行分析。

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-10-08 DOI:10.3390/bios14100487
Ana Filipa R Cerqueira, Catarina Moreirinha, Mariana Raposo, Maria Teresa S R Gomes, Sara T Costa, Maria João Botelho, Alisa Rudnitskaya
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引用次数: 0

摘要

利用电位化学传感器开发了一种简单可靠的方法来检测双壳贝类组织中的麻痹性贝类毒素(PST)。评估了从贻贝和牡蛎组织中提取 PST 的五种方法,包括 AOAC 推荐的参考方法。主要目的是尽量减少提取物对传感器响应的基质效应,并确保有效的毒素回收。使用醋酸加热和水进行提取的程序使电位化学传感器对 PST 的反应最高。使用醋酸和加热萃取法从双壳类组织中回收的 PST 最高。进一步的提取纯化对于液相色谱-荧光检测(LC-FLD)分析是必不可少的,但对于使用化学传感器进行分析则没有必要。虽然水提取也可作为一种快速、简单的 PST 提取方法,但在解释结果时应考虑到较低的回收率。需要进一步研究,以确定萃取物中残留的导致传感器响应降低的化合物,并制定消除这些化合物的程序。
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Paralytic Shellfish Toxin Extraction from Bivalve Meat for Analysis Using Potentiometric Chemical Sensors.

A simple and reliable methodology for the detection of paralytic shellfish toxins (PSTs) in bivalve tissues using potentiometric chemical sensors was developed. Five methods of PST extraction from mussel and oyster tissues were evaluated, including the AOAC-recommended method, which served as the reference. The main objective was to minimize the matrix effect of the extracts on the sensors' responses and ensure efficient toxin recovery. Extraction procedures using acetic acid with heating and water yielded the highest responses from the potentiometric chemical sensors to PSTs. The highest recovery of PSTs from bivalve tissues was achieved with extraction using acetic acid and heating. Further extract purification, which is indispensable for liquid chromatography with fluorometric detection (LC-FLD) analysis, was found to be unnecessary for analysis with chemical sensors. While water extraction can also be used as a rapid and simple PST extraction method, the lower recoveries should be considered when interpreting the results. Further research is needed to identify the compounds remaining in the extracts that cause a decrease in sensor responses and to develop procedures for their elimination.

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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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