艾灸通过Tim-3/Gal-9信号通路调控类风湿性关节炎的T细胞表达

Rejuvenation research Pub Date : 2025-02-01 Epub Date: 2024-10-24 DOI:10.1089/rej.2024.0052
Yu-Mei Zhong, Kun Luo, Yan-Ding Guo, Xiu-Hua Gao, Hai-Yan Zhou
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引用次数: 0

摘要

观察艾灸对类风湿性关节炎(RA)大鼠T细胞及T细胞免疫球蛋白和含粘蛋白域分子-3/galectin-9(Tim-3/Gal-9)通路的影响。进一步探讨艾灸治疗 RA 的可能抗炎机制。研究人员将 30 只 Sprague Dawley 大鼠随机分为三组,包括对照组、RA 模型组和艾灸组。对照组通过注射弗氏完全佐剂建立 RA 模型。艾灸组对大鼠进行 "神阙 "和 "足三里 "穴位的艾灸治疗。共治疗三个疗程。然后测量足垫厚度,用苏木精-伊红(HE)染色观察关节病理变化,用流式细胞仪检测外周血中 CD4+T 和 CD8+T 的比例,用聚合酶链式反应(PCR)检测滑膜中 Tim-3 和 Gal-9 的表达水平,用免疫荧光检测滑膜中 CD4+T 和 CD8+T 的表达。HE 染色显示,对照组滑膜组织光滑,排列整齐,无炎症细胞浸润。模型组关节间隙变窄,滑膜组织充血水肿,大量炎性细胞浸润。与模型组相比,艾灸组的关节间隙变窄,滑膜充血水肿,炎性细胞浸润程度降低。流式细胞术显示,与模型组相比,艾灸组 CD4+T 表达下调,而 CD8+T 表达上调。PCR 结果显示,与模型组相比,艾灸组的 Tim-3 和 Gal-9 表达上调。免疫荧光结果显示,与模型组相比,艾灸组 CD4+T 表达降低,而 CD8+T 表达升高。结果表明,艾灸不仅能抑制 CD4+T 的表达,还能促进 CD8+T 的表达。艾灸的抗炎作用可能与通过 Tim-3/Gal-9 信号通路调节 T 细胞表达有关。
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Moxibustion Regulates the Expression of T Cells in Rheumatoid Arthritis Through Tim-3/Gal-9 Signaling Pathway.

To observe the effects of moxibustion on T cells and T cell immunoglobulin and mucin-domain-containing molecule-3/galectin-9 (Tim-3/Gal-9) pathway in rats with rheumatoid arthritis (RA). To further explore the possible anti-inflammatory mechanism of moxibustion in the treatment of RA. Thirty Sprague Dawley rats were randomly divided into three groups, including a control group, an RA model group, and a moxibustion group. An RA model was created through the injection of Freund's complete adjuvant. In the moxibustion group, rats were treated with moxibustion at acupoints of "Shenshu" and "Zusanli." A total of three courses of treatment were conducted. Then the thickness of foot pad was measured, joint pathological changes were observed by hematoxylin-eosin (HE) staining, the proportion of CD4+T and CD8+T in peripheral blood was detected by flow cytometry, the expression levels of Tim-3 and Gal-9 in synovium were detected by polymerase chain reaction (PCR), and the expressions of CD4+T and CD8+T in synovium were detected by immunofluorescence. HE staining showed that the synovial tissue of the control group was smooth and neatly arranged without inflammatory cell infiltration. In the model group, the joint space was narrowed, the synovial tissue had congestion and edema, and a large number of inflammatory cells infiltrated. Compared with the model group, in the moxibustion group, the joint space narrowed with synovium hyperemia and edema, and the level of inflammatory cell infiltration decreased. Flow cytometry showed that compared with the model group, CD4+T expression in the moxibustion group was downregulated, while CD8+T expression was upregulated. PCR results showed that compared with the model group, the expressions of Tim-3 and Gal-9 in the moxibustion group were upregulated. Immunofluorescence results showed that compared with the model group, CD4+T expression in the moxibustion group was decreased, while CD8+T expression was increased. The results demonstrate that moxibustion not only suppressed the expression of CD4+T but also promoted the expression of CD8+T. The anti-inflammatory effect of moxibustion may be related to the regulation of T cell expression through the Tim-3/Gal-9 signaling pathway.

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Relationship of α-Klotho with Frailty Index and Sarcopenia: A Bidirectional Mendelian Randomization Study. Related Factors with Vascular Dementia: A Two-Sample Mendelian Randomization Study. Moxibustion Regulates the Expression of T Cells in Rheumatoid Arthritis Through Tim-3/Gal-9 Signaling Pathway. Parishin Alleviates Pulmonary Fibrosis by Reducing CD38 Levels in Naturally Aging Mice. Correction to: Dietary Restriction Attenuates Inflammation and Protects Mouse Skin from High-Dose Ultraviolet B Irradiation (doi: 10.1089/rej.2021.0022).
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