优化萃取以高效回收剑麻籽蛋白质分离物：评估理化和技术功能特性。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2024-10-29 DOI:10.1002/jsfa.13979

Nur Aqilah Hamim, Nazamid Saari, Wan Zunairah Wan Ibadullah, Abdul Manan Dos Mohamed, Farooq Anwar, Muhamad Yusuf Hasan, Mohd Zulkhairi Abdul Rahim, Muhammad Sharir Abdul Rahman, Roselina Karim

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引用次数: 0

摘要

背景：洋麻种子是一种丰富的蛋白质来源；然而，要从这些未充分利用的种子中获得优质蛋白质，找到最佳提取方法至关重要。本研究采用响应面方法设计了一种优化提取工艺，以获得最佳的剑麻籽蛋白质回收率。对影响产量和蛋白质含量的关键参数进行了优化，包括提取 pH 值（2-11）、种子与水的比例（5:1-50:1）、温度（30-90 °C）和持续时间（20-360 分钟）。结果显示，最高蛋白质产量为 12.05%：在 pH 值为 11.0、种子与水的比例为 50:1、萃取时间为 360 分钟、温度为 50 ℃的优化萃取条件下，蛋白质的最高产量为 12.05 克/100 克，纯度为 91.94 克/100 克。KSPI 的保油量和保水量分别为 1.14 mL g-1 和 1.37 mL g-1。在 pH 值为 7 的条件下 30 分钟后，KSPIs 表现出显著的乳化能力（83.12%）和稳定性（75.63%），以及较高的发泡能力（106%）和稳定性（18.3%）。根据高效液相色谱分析，精氨酸、谷氨酸、亮氨酸、苯丙氨酸和赖氨酸是在 KPSIs 中检测到的最丰富的氨基酸。傅立叶变换红外光谱、蛋白质组分比率和差示扫描量热法等分析方法成功验证了 KSPIs 的球状蛋白质结构。十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析表明，KPSI 的分子量分布在 10 kDa 至 50 kDa 之间：本研究的结果支持应用所提出的响应面方法优化萃取方法，以高效回收符合必要理化和技术功能要求的优质剑麻籽蛋白。© 2024 化学工业学会。

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Optimization of extraction for efficient recovery of kenaf seed protein isolates: evaluation of physicochemical and techno-functional characteristics.

Background: Kenaf seeds are a rich source of protein; however, finding the best extraction method is crucial to obtaining high-quality protein from these underutilized seeds. This research devised an optimized extraction process for best recovery of kenaf seeds protein using response surface methodology. The key parameters affecting the yield and protein content were optimized, including extraction pH (2-11), seed:water ratio (5:1-50:1), temperature (30-90 °C), and duration (20-360 min). The physicochemical and techno-functional properties of kenaf seed protein isolates (KSPIs) were examined.

Results: A maximum protein yield of 12.05 g/100 g with purity level 91.94 g/100 g was obtained using an optimized extraction with pH 11.0, seed:water ratio 50:1, 360 min duration, and temperature 50 °C. The oil and water retention capacities of KSPI were 1.14 mL g^-1 and 1.37 mL g^-1 respectively. After 30 min at pH 7, KSPIs demonstrated remarkable emulsion capacity (83.12%) and stability (75.63%), along with high foaming capacity (106%) and stability (18.3%). As per high-performance liquid chromatography analysis, arginine, glutamic acid, leucine, phenylalanine, and lysine were the most abundant amino acids detected in KPSIs. The KSPIs' globular protein structure was successfully verified using analytical approaches, including Fourier transform infrared spectroscopy, protein fraction ratios, and differential scanning calorimetry. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis revealed that KPSI has a molecular weight distribution ranging from 10 kDa to 50 kDa.

Conclusion: The results of this study support the application of the proposed response-surface-methodology-optimized extraction method for efficient recovery of high-quality kenaf seed proteins that meet the necessary physicochemical and techno-functional requirements. © 2024 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.