A comprehensive review on the pretreatment and detection methods of nitrofurans and their metabolites in animal-derived food and environmental samples

In recent years, the residues of nitrofurans (NFs) and their metabolites in animal-derived food and environmental samples have gained widespread attention. The parent drugs and their metabolites have displayed significant toxicity to human health including carcinogenic, mutagenic and teratogenic effects, leading to banned in animal husbandry in many countries. Hence, monitoring the residues of NFs is necessary to guarantee public health and ecological security. This review aims to summarize and assess the structural properties, residue status, sample pretreatment methods (liquid-liquid extraction, solid-phase extraction, Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS), and field-assisted extraction), and detection methods (chromatographic analysis, immunoassay, and some innovative detection methods) for NFs and their metabolites in animal-derived food and environmental samples. This paper provides a detailed reference and discussion for the analysis of NFs and their metabolites, which can effectively promote the establishment of innovative detection methods for NFs and their metabolites residues.