Rapid detection of chlorpyrifos in miscellaneous beans based on nitrogen and phosphorus doped carbon quantum dots fluorescence probe

A fluorescent probe based on N, P-doped carbon quantum dots was established to detect chlorpyrifos. N, P-doped carbon quantum dots were prepared by one-step hydrothermal method with trisodium citrate, dipotassium hydrogen phosphate and urea. The sensing mechanism was based on the fluorescence quenching of N, P-doped carbon quantum dots by chlorpyrifos through internal filtering effect. The results indicated that the linear range was 0.025 ∼ 25 µg/mL with the detection limit of 0.0008 µg/g under the optimal detection conditions. The method was applied to different miscellaneous beans with the recoveries of 90.2∼105.7 % and the relative standard deviations were less than 4.8 %. The fluorescent probe has the advantages of economy, high efficiency, easy preparation and specificity.