确定三疣藻非光化学淬火逆转的基因。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-10-30 DOI:10.1111/tpj.17104
Maxwell A Ware, Andrew J Paton, Yu Bai, Tessema Kassaw, Martin Lohr, Graham Peers
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引用次数: 0

摘要

硅藻和七鳃鳗等藻类具有不同于植物的光合色素,包括一套新颖的类胡萝卜素。其中包括一个由二二黄原酸及其去氧化产物二氧黄原酸组成的初级黄绿素循环,该循环可在光收集和非光化学淬灭(NPQ)介导的光能耗散之间切换。负责这一循环逆转的酶以前并不为人所知。在这里,我们从三疣藻中发现了玉米黄质环氧化酶 3(ZEP3),作为候选的二氧黄质环氧化酶。敲除 ZEP3 基因会导致饱和光照下快速可逆的 NPQ 损失。这与在弱光下恢复期间维持高浓度的二氧黄素有关。通过与野生型 ZEP3 基因互补,黄绿素循环和 NPQ 松弛得以恢复。zep3基因敲除菌株在较高光通量下光合速率降低,在多变光照条件下特定生长速率降低,这可能是由于突变菌株锁定在光能耗散状态。通过将 ZEP3 基因置于β-雌二醇诱导启动子的控制下,我们能够以时间和剂量依赖的方式切换 NPQ 能力水平。该基因的鉴定加深了人们对藻类光合作用控制的多样性的理解,并为提高工业规模培养的生产力提供了一个潜在的目标。
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Identifying the gene responsible for non-photochemical quenching reversal in Phaeodactylum tricornutum.

Algae such as diatoms and haptophytes have distinct photosynthetic pigments from plants, including a novel set of carotenoids. This includes a primary xanthophyll cycle comprised of diadinoxanthin and its de-epoxidation product diatoxanthin that enables the switch between light harvesting and non-photochemical quenching (NPQ)-mediated dissipation of light energy. The enzyme responsible for the reversal of this cycle was previously unknown. Here, we identified zeaxanthin epoxidase 3 (ZEP3) from Phaeodactylum tricornutum as the candidate diatoxanthin epoxidase. Knocking out the ZEP3 gene caused a loss of rapidly reversible NPQ following saturating light exposure. This correlated with the maintenance of high concentrations of diatoxanthin during recovery in low light. Xanthophyll cycling and NPQ relaxation were restored via complementation of the wild-type ZEP3 gene. The zep3 knockout strains showed reduced photosynthetic rates at higher light fluxes and reduced specific growth rate in variable light regimes, likely due to the mutant strains becoming locked in a light energy dissipation state. We were able to toggle the level of NPQ capacity in a time and dose dependent manner by placing the ZEP3 gene under the control of a β-estradiol inducible promoter. Identification of this gene provides a deeper understanding of the diversification of photosynthetic control in algae compared to plants and suggests a potential target to improve the productivity of industrial-scale cultures.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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