将植物中的伯克霍尔德氏菌(Burkholderia ambifaria)菌株作为一种新型益生菌用于龋齿防治的评估。

IF 3.7 2区 医学 Q2 MICROBIOLOGY Journal of Oral Microbiology Pub Date : 2024-11-03 eCollection Date: 2024-01-01 DOI:10.1080/20002297.2024.2420612
Zirang Chen, Yangyu Lu, Zhezhen Xu, Lijing Wu, Xi Wei, Yanling Cai
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引用次数: 0

摘要

背景:益生菌能够逆转菌群失调,恢复健康的微生物群,因此是一种新型的龋齿预防或治疗方法。在此,我们发现了一种从植物中分离出来的候选益生菌--安氏伯克霍尔德氏菌(Burkholderia ambifaria AFS098024):方法:通过溶血活性、D-乳酸产量和抗生素敏感性来评估安氏伯克霍尔德氏菌的安全性。方法:通过溶血活性、D-乳酸产生量和抗生素敏感性对伏地菌的安全性进行评估,并使用无龋和有龋供体唾液中的体外生物膜模型和体内大鼠龋模型来评估伏地菌在龋齿预防和治疗中的功效:结果:B. ambifaria作为候选益生菌是安全的,它能与体外生物膜模型相结合。它能明显减少龋齿活性供体生物膜的生物量和乳酸盐产量,并破坏生物膜结构。无论接种顺序如何,伏地芽孢杆菌都能有效降低大鼠臼齿龋齿的严重程度。经过预处理或使用伏地芽孢杆菌处理的臼齿的珐琅质体积明显增大。此外,埋伏噬菌体在大鼠臼齿中的定植可持续 6 周:结论:本研究中使用的埋伏芽孢杆菌菌株有望成为一种在体外和体内抑制龋齿的益生菌。
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Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management.

Background: Probiotics serve as a novel preventive or therapeutic approach for dental caries owing to their ability to reverse dysbiosis and restore a healthy microbiota. Here, we identified Burkholderia ambifaria AFS098024 as a probiotic candidate isolated from plants.

Methods: The safety of B. ambifaria was evaluated by hemolytic activity, D-lactic acid production and antibiotic susceptibility. In vitro biofilm model derived from the saliva of caries-free and caries-active donors and in vivo rat caries model were used to assess the efficacy of B. ambifaria in caries prevention and treatment.

Results: B. ambifaria was safe as a probiotic candidate and it could integrate with in vitro biofilm model. It significantly reduced the biomass and lactate production of biofilms from caries-active donors and disrupted biofilm structures. B. ambifaria effectively reduced the severity of carious lesions in rat molars, regardless of the inoculation sequence. Molars pretreated or treated with B. ambifaria demonstrated notably higher enamel volumes. Additionally, colonization of rat molars by B. ambifaria persisted for 6 weeks.

Conclusion: The B. ambifaria strain used in this study holds promise as a probiotic for inhibiting dental caries, both in vitro and in vivo.

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来源期刊
CiteScore
8.00
自引率
4.40%
发文量
52
审稿时长
12 weeks
期刊介绍: As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies. Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics. Article types: original articles, notes, review articles, mini-reviews and commentaries
期刊最新文献
Oral administration of Porphyromonas gingivalis to mice with diet-induced obesity impairs cognitive function associated with microglial activation in the brain. RE: colonization of bacterial and viral respiratory pathogens among healthcare workers in China during COVID-19 pandemic. In vitro plaque formation model to unravel biofilm formation dynamics on implant abutment surfaces. Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management. Methanobrevibacter oralis: a comprehensive review.
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