纳豆芽孢杆菌固态发酵在氨氮移动和土壤保水过程中产生聚-γ-谷氨酸。

IF 3.4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Enzyme and Microbial Technology Pub Date : 2024-10-31 DOI:10.1016/j.enzmictec.2024.110543
Xin Wang, Jie Gao, Jiahui Wu, Xuan Li, Junxun Li, Haihong Li, Songlin Wang
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引用次数: 0

摘要

通过紫外线诱变处理筛选出一株高产聚谷氨酸(γ-PGA)的纳豆芽孢杆菌 UV-40-50 株,并确定其仍属于芽孢杆菌属。通过单因素方差分析确定了纳豆芽孢杆菌 UV-40-50 菌株固态发酵(SSF)的最佳发酵培养基组成,在此条件下,γ-PGA 的产量为 55.19 g/kg,并通过一系列表征确定了γ-PGA 纯化样品中γ-PGA 的存在和分子量。进一步研究了未分离固体发酵产物(SFP)对水体中氨氮和亚硝酸盐的净化能力,以及对土壤保水性、莴苣和卷心菜发芽率和苗长的影响。结果表明,添加 1 克/立方米的 SFP 能有效去除水体中 60% 以上的氨氮和 40% 以上的亚硝酸盐;添加 0.01% 的 SFP 能使白菜土壤的保水能力提高 2.13 倍,使莴苣土壤的保水能力提高 12%;同时,SFP 还能显著提高白菜和莴苣的发芽率和苗长。
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Poly-γ-glutamic production by solid-state fermentation of Bacillus natto in ammonia nitrogen movement and soil water retention processes.

A high polyglutamic acid (γ-PGA) producing strain of Bacillus natto UV-40-50 was screened by ultraviolet mutagenesis treatment and identified as still belonging to the Bacillus specie. The optimal fermentation medium composition for solid state fermentation (SSF) of B. natto strain UV-40-50 strain was determined by one-way analysis of variance, under which the yield of γ-PGA was 55.19 g/kg, and the presence and molecular weight of γ-PGA in the γ-PGA-purified samples were determined by a series of characterizations. The purification ability of the unseparated solid fermentation product (SFP) on ammonia nitrogen and nitrite in the water column, as well as its effect on soil water retention, germination rate and seedling length of lettuce and cabbage were further investigated. The results showed that the addition of 1 g/m3 SFP could effectively remove more than 60 % of ammonia nitrogen and more than 40 % of nitrite in the water body; the addition of 0.01 % SFP could increase the water retention capacity of cabbage soil by 2.13 times, and increase the water retention capacity of lettuce soil by 12 %; at the same time, the SFP could also significantly increase the germination rate and seedling length of both cabbage and lettuce.

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来源期刊
Enzyme and Microbial Technology
Enzyme and Microbial Technology 生物-生物工程与应用微生物
CiteScore
7.60
自引率
5.90%
发文量
142
审稿时长
38 days
期刊介绍: Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.
期刊最新文献
Editorial Board Poly-γ-glutamic production by solid-state fermentation of Bacillus natto in ammonia nitrogen movement and soil water retention processes. Advances and prospects for lactic acid production from lignocellulose The synthesis of cinnamyl acetate and deacetyl-7-aminocephalosporanic acid by a GDSL-type esterase and its substrate specificity analysis Spectrophotometric assay for the screening of selective enzymes towards DHA and EPA ethyl esters hydrolysis
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