对老化小鼠群落进行家庭笼舍自动监测--对福利监测和实验的启示

IF 3.2 3区 医学 Q2 NEUROSCIENCES Frontiers in Neuroscience Pub Date : 2024-10-29 eCollection Date: 2024-01-01 DOI:10.3389/fnins.2024.1489308
Joanna L Moore, James Kennedy, Abdul-Azim Hassan
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引用次数: 0

摘要

介绍:我们对实验动物的行为以及饲养活动对其健康的影响的了解仍不全面。这一点与老龄化鼠群尤其相关,因为它们的活动模式可能会随着成熟而改变。家庭笼养监测(HCM)为了解小鼠在动物自身环境中的活动提供了宝贵的信息,并能揭示适应期和对饲养活动(如换笼)的反应。本研究的目的是监测和探索老龄雌雄 C57/BL6 小鼠群体的活动和休息干扰(RDI)模式的变化:方法:将小鼠饲养在数字通风笼®系统中长达18个月。然后下载数据,研究小鼠的活动模式和 RDI 随时间的变化情况。习惯化、老化和笼子变化评估采用线性混合模型进行,而笼子分离和刻板行为调查则通过目测数据进行:正如研究期间所预期的那样,与黑暗阶段相比,小鼠在光照阶段的活动较少。然而,小鼠到达后,在光照阶段表现出更高的活动量和RDI,而在黑暗阶段则表现出更低的活动量和RDI,需要几天时间才能调整到基线 "适应 "模式。随着年龄的增长,小鼠的总体活动量在 5 个月到 14 个月期间明显减少,之后又恢复到基线水平。我们在对该鸟群进行监测时还观察到了活动高峰。长期饲养会导致动物出现令人震惊的刻板行为。被标记为可能出现刻板行为的小鼠笼子在光照和黑暗阶段都显示出持续的活动尖峰。黑暗阶段的活动峰值比光照阶段明显得多。与前一天相比,换笼会导致明相活动和 RDI 的增加,而在换笼后的暗相活动中没有观察到差异。随着动物年龄的增长,这种效应保持不变:本研究探讨了饲养在数字通风笼®系统中的雌雄 C57/BL6 小鼠老化群体活动模式的变化。我们确定了有关活动和 RDI 差异的不同衰老阶段,以及 DVC® 的潜在新福利应用,特别是用于早期检测刻板行为。总之,从福利和行为的角度来看,长期饲养动物时应考虑采用 HCM 系统。
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Automated home cage monitoring of an aging colony of mice-Implications for welfare monitoring and experimentation.

Introduction: Our understanding of laboratory animal behavior and the implications of husbandry activities on their wellbeing remains incomplete. This is especially relevant with an aging colony as their activity patterns may change as they mature. Home Cage Monitoring (HCM) provides valuable insights into mouse activity within the animal's own environment and can shed light on acclimatization periods and responses to husbandry activities such as cage changing. The aim of this study was to monitor and explore changes in the activity and rest disturbance (RDI) patterns of an aging colony of male and female C57/BL6 mice.

Methods: The mice were housed in the Digitally Ventilated Cage® system, for up to 18 months of age. Data was then downloaded to investigate how the activity patterns and RDI of the mice changed over time. Habituation, aging and cage change assessments were conducted using linear mixed models, while cage separation and stereotypic behavior investigations were conducted by visual inspection of the data.

Results: As expected during the study, mice were less active during the light phase compared to the dark phase. However, on arrival mice displayed heightened activity and RDI during the light phase and reduced activity and RDI during the dark phase, taking several days to adjust to baseline "acclimatized" patterns. With age, overall activity significantly decreased from 5 months until 14 months of age, after which it increased back toward baseline levels. We also observed activity spikes during our monitoring of this colony. Prolonged housing can lead to alarming stereotypic behaviors in animals. Cages of mice flagged for potential stereotypy displayed sustained activity spikes in the light and dark phases. Spikes in activity during the dark phase were much more pronounced than in the light phase. Cage changing led to an increase in the light phase activity and RDI compared to the previous day, with no observed difference in the dark phase post-cage change. This effect remained consistent as the animals aged.

Discussion: This study explores changes in the activity patterns of an aging colony of male and female C57/BL6 mice housed in the Digitally Ventilated Cage® system. We identified distinct aging phases concerning activity and RDI differences and a potential new welfare application for the DVC®, specifically for early detection of stereotypy. In conclusion, the adoption of HCM systems should be considered for long-term animal housing from both a welfare and behavioral perspective.

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来源期刊
Frontiers in Neuroscience
Frontiers in Neuroscience NEUROSCIENCES-
CiteScore
6.20
自引率
4.70%
发文量
2070
审稿时长
14 weeks
期刊介绍: Neural Technology is devoted to the convergence between neurobiology and quantum-, nano- and micro-sciences. In our vision, this interdisciplinary approach should go beyond the technological development of sophisticated methods and should contribute in generating a genuine change in our discipline.
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