tsaoko条纹镶嵌病毒的完整基因组序列，这是一种在Amomum tsaoko中发现的新型巨型病毒

IF 2.5 4区医学 Q3 VIROLOGY Archives of Virology Pub Date : 2024-11-15 DOI:10.1007/s00705-024-06177-2

Xiyu Yu, Xiaodie Zou, Lei Zhang, Lianzhang Wu, Yi Yang, Guodong Li, Jiahong Dong

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引用次数: 0

摘要

通过高通量测序，在Aomum tsaoko中发现了一种新型巨细胞病毒，暂命名为 "tsaoko条纹花叶病毒"（TkSMV）。利用 RT-PCR 和 RACE 测定了 TkSMV 的完整基因组序列。该基因组序列由 8218 个核苷酸组成（不包括聚（A）尾），包含一个大的开放阅读框，编码 2625 个氨基酸的多聚蛋白，分子量约为 297.13 kDa。TkSMV 与 Alpinia oxyphylla 镶嵌病毒的亲缘关系最密切，核苷酸和氨基酸序列的一致性分别为 71.5%和 75.9%。这些数值低于 Potyviridae 科的物种划分阈值。这些结果表明，TkSMV 应被视为 Macluravirus 属的一个独特成员。

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Complete genome sequence of tsaoko stripe mosaic virus, a novel macluravirus found in Amomum tsaoko

A novel macluravirus, tentatively named "tsaoko stripe mosaic virus" (TkSMV), was identified in Amomum tsaoko through high-throughput sequencing. The complete genome sequence of TkSMV was determined using RT-PCR and RACE. The genome sequence consists of 8218 nucleotides, excluding the poly(A) tail, and contains a large open reading frame encoding a polyprotein of 2625 amino acids with a molecular weight of approximately 297.13 kDa. TkSMV is most closely related to Alpinia oxyphylla mosaic virus, sharing 71.5% nucleotide and 75.9% amino acid sequence identity. These values are below the species demarcation threshold for the family Potyviridae. These results suggest that TkSMV should be considered a distinct member of the genus Macluravirus.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.