Yun Ji Park, Jae Kwang Kim, Nam Su Kim, Young Jin Park, Ramaraj Sathasivam, Sang Un Park
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引用次数: 0
摘要
背景光是植物生长发育中的一个重要因素，对植物的初级和次级新陈代谢都有重大影响。药蜀葵（Althaea officinalis）俗称棉花糖，其叶子和根部具有多种用途。由于它具有大量已确定的生物活性化合物，并广泛用于食品、保健品和补充剂，因此在全球范围内被广泛种植。本研究旨在通过综合代谢组和转录组分析，证明黑暗和光照对 A. officinalis 毛根的初级和次级代谢物产生的明确积极影响，并阐明光反应机制。多元统计分析显示，光照和黑暗处理的毛细根之间存在明显的差异，这可能归因于谷氨酸、苯丙氨酸、儿茶素水合物和叶绿素等代谢物。相应地，受到显著影响的途径包括半乳糖代谢、丙氨酸、天门冬氨酸和谷氨酸代谢、黄酮和黄酮醇生物合成以及苯丙氨酸代谢。通过 RNA 测序分析和比较了与色素和苯丙氨酸生物合成途径相关的光响应差异表达基因。此外，在与光有关的转录因子中，负责光形态调控的 CONSTANS-LIKE 和双 B-box 锌指等转录因子都出现了上调。此外，核酮糖二磷酸羧化酶、光系统 II 和叶绿素 A-B 结合家族蛋白等光反应基因也被上调。
Integrated metabolome and transcriptome analyses provide comprehensive insight into dark- and light-responsive mechanisms in Althaea officinalis hairy root cultures
Background
Light, an essential factor in plant development, exerts a significant impact on both primary and secondary metabolism in plants. Althaea officinalis, commonly known as marshmallow, offers versatile applications through its leaves and roots. With a plethora of identified bioactive compounds and their extensive use in food, health, and supplements, it is widely cultivated globally. This study aimed to demonstrate the definitive positive impact of dark and light irradiation on both primary and secondary metabolite production in A. officinalis hairy roots and to elucidate the light-responsive mechanism through integrated metabolome and transcriptome analysis.
Results
When exposed to light, significant changes with a greenish colour shift were observed in 60 metabolites. Multivariate statistical analysis revealed a distinct separation between light- and dark-treated hairy roots, likely attributed to metabolites such as glutamic acid, phenylalanine, catechin hydrate, and chlorophyll. Correspondingly, the pathways significantly impacted included galactose metabolism, alanine, aspartate, and glutamate metabolism, flavone and flavonol biosynthesis, and phenylalanine metabolism. Light-responsive differentially expressed genes associated with pigment and phenylpropanoid biosynthetic pathways were analysed and compared via RNA sequencing. Furthermore, among the light-related transcription factors, including CONSTANS-LIKE and double B-box zinc finger, which are responsible for photomorphogenic modulation, were upregulated. Moreover, light-responsive genes, such as ribulose bisphosphate carboxylase, photosystem II, and chlorophyll A-B binding family protein, were upregulated.
Conclusions
These findings emphasise that exposure of A. officinalis hairy root culture to light conditions is a useful method for enhancing most of the primary and secondary metabolites.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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