D. Semeshchenko , M.F. Veiga , M. Visus , A. Farinati , I. Huespe , HIBA Hip Surgery Unit , M.A. Buttaro , P.A. Slullitel
{"title":"聚维酮碘和硝酸银对消除钛表面葡萄球菌生物膜同样有效：体外分析","authors":"D. Semeshchenko , M.F. Veiga , M. Visus , A. Farinati , I. Huespe , HIBA Hip Surgery Unit , M.A. Buttaro , P.A. Slullitel","doi":"10.1016/j.jhin.2024.11.012","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>There is no consensus on the irrigation solution and concentration that should be used when performing a debridement, antibiotics, and implant retention (DAIR) surgery.</div></div><div><h3>Aim</h3><div>To determine the minimum biofilm eradication concentration (MBEC) of five antibacterial solutions and to compare their efficacies in eradicating staphylococcal biofilm embedded on a titanium surface.</div></div><div><h3>Methods</h3><div>Meticillin-sensitive <em>Staphylococcus aureus</em> (MSSA) and meticillin-resistant <em>S. aureus</em> (MRSA) ATCC standard strains were grown over porous Ti–6Al–4V acetabular screw-caps. Antibacterial solutions were povidone-iodine, rifampicin, silver nitrate, copper sulphate, and chlorhexidine. MBEC values were calculated for MSSA and MRSA. After 24 h, screw-caps were exposed for 3 min to each solution. Bacterial separation from each specimen was performed with vortex agitation and footprint on agar plate in triplicate. Colony forming units (cfu) were counted pre- and post-agitation, and the delta of cfu/mL was calculated for each solution. A threefold log reduction in cfu was considered a measure of solution efficacy. Comparison between groups was made with Fisher's test.</div></div><div><h3>Findings</h3><div>MBEC values for MSSA and MRSA, respectively, were as follows: 8000 μg/mL and 16,000 μg/mL for povidone-iodine; 64 μg/mL and 128 μg/mL for rifampicin; 10,000 μg/mL and 5120 μg/mL for silver nitrate; 900 μg/mL and 900 μg/mL for copper sulphate; 16 μg/mL and 32 μg/mL for chlorhexidine. Rifampicin, copper sulphate and chlorhexidine were ineffective against MSSA and MRSA biofilm compared with povidone-iodine (<em>P</em><0.01) and silver nitrate (<em>P</em>=0.015) that had a delta cfu reduction >8 log. Povidone-iodine and silver nitrate showed negative footprints without visible MSSA (<em>P</em>=0.005) and MRSA (<em>P</em>=0.014).</div></div><div><h3>Conclusions</h3><div>Povidone-iodine and silver nitrate were the only irrigating solutions capable of eradicating at least 99.9% of 24-h biofilm.</div></div>","PeriodicalId":54806,"journal":{"name":"Journal of Hospital Infection","volume":"155 ","pages":"Pages 185-191"},"PeriodicalIF":3.9000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Povidone-iodine and silver nitrate are equally effective in eradicating staphylococcal biofilm grown on a titanium surface: an in-vitro analysis\",\"authors\":\"D. Semeshchenko , M.F. Veiga , M. Visus , A. Farinati , I. Huespe , HIBA Hip Surgery Unit , M.A. Buttaro , P.A. Slullitel\",\"doi\":\"10.1016/j.jhin.2024.11.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>There is no consensus on the irrigation solution and concentration that should be used when performing a debridement, antibiotics, and implant retention (DAIR) surgery.</div></div><div><h3>Aim</h3><div>To determine the minimum biofilm eradication concentration (MBEC) of five antibacterial solutions and to compare their efficacies in eradicating staphylococcal biofilm embedded on a titanium surface.</div></div><div><h3>Methods</h3><div>Meticillin-sensitive <em>Staphylococcus aureus</em> (MSSA) and meticillin-resistant <em>S. aureus</em> (MRSA) ATCC standard strains were grown over porous Ti–6Al–4V acetabular screw-caps. Antibacterial solutions were povidone-iodine, rifampicin, silver nitrate, copper sulphate, and chlorhexidine. MBEC values were calculated for MSSA and MRSA. After 24 h, screw-caps were exposed for 3 min to each solution. Bacterial separation from each specimen was performed with vortex agitation and footprint on agar plate in triplicate. Colony forming units (cfu) were counted pre- and post-agitation, and the delta of cfu/mL was calculated for each solution. A threefold log reduction in cfu was considered a measure of solution efficacy. Comparison between groups was made with Fisher's test.</div></div><div><h3>Findings</h3><div>MBEC values for MSSA and MRSA, respectively, were as follows: 8000 μg/mL and 16,000 μg/mL for povidone-iodine; 64 μg/mL and 128 μg/mL for rifampicin; 10,000 μg/mL and 5120 μg/mL for silver nitrate; 900 μg/mL and 900 μg/mL for copper sulphate; 16 μg/mL and 32 μg/mL for chlorhexidine. Rifampicin, copper sulphate and chlorhexidine were ineffective against MSSA and MRSA biofilm compared with povidone-iodine (<em>P</em><0.01) and silver nitrate (<em>P</em>=0.015) that had a delta cfu reduction >8 log. 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Povidone-iodine and silver nitrate are equally effective in eradicating staphylococcal biofilm grown on a titanium surface: an in-vitro analysis
Background
There is no consensus on the irrigation solution and concentration that should be used when performing a debridement, antibiotics, and implant retention (DAIR) surgery.
Aim
To determine the minimum biofilm eradication concentration (MBEC) of five antibacterial solutions and to compare their efficacies in eradicating staphylococcal biofilm embedded on a titanium surface.
Methods
Meticillin-sensitive Staphylococcus aureus (MSSA) and meticillin-resistant S. aureus (MRSA) ATCC standard strains were grown over porous Ti–6Al–4V acetabular screw-caps. Antibacterial solutions were povidone-iodine, rifampicin, silver nitrate, copper sulphate, and chlorhexidine. MBEC values were calculated for MSSA and MRSA. After 24 h, screw-caps were exposed for 3 min to each solution. Bacterial separation from each specimen was performed with vortex agitation and footprint on agar plate in triplicate. Colony forming units (cfu) were counted pre- and post-agitation, and the delta of cfu/mL was calculated for each solution. A threefold log reduction in cfu was considered a measure of solution efficacy. Comparison between groups was made with Fisher's test.
Findings
MBEC values for MSSA and MRSA, respectively, were as follows: 8000 μg/mL and 16,000 μg/mL for povidone-iodine; 64 μg/mL and 128 μg/mL for rifampicin; 10,000 μg/mL and 5120 μg/mL for silver nitrate; 900 μg/mL and 900 μg/mL for copper sulphate; 16 μg/mL and 32 μg/mL for chlorhexidine. Rifampicin, copper sulphate and chlorhexidine were ineffective against MSSA and MRSA biofilm compared with povidone-iodine (P<0.01) and silver nitrate (P=0.015) that had a delta cfu reduction >8 log. Povidone-iodine and silver nitrate showed negative footprints without visible MSSA (P=0.005) and MRSA (P=0.014).
Conclusions
Povidone-iodine and silver nitrate were the only irrigating solutions capable of eradicating at least 99.9% of 24-h biofilm.
期刊介绍：
The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience.
The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that:
provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings;
provide new insight into cleaning, disinfection and decontamination;
provide new insight into the design of healthcare premises;
describe novel aspects of outbreaks of infection;
throw light on techniques for effective antimicrobial stewardship;
describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control;
improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change;
improve understanding of the use of IT systems in infection surveillance and prevention and control.
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