{"title":"硅酸盐对骨骼水蚤多胺氧化酶基因的影响","authors":"Wei Teng , Xiaomei Shang , Jun Sun","doi":"10.1016/j.marenvres.2024.106860","DOIUrl":null,"url":null,"abstract":"<div><div>By using <em>Skeletonema dohrnii</em> as the experimental algal species, we investigated how silicate concentration impacts the polyamine metabolism of diatoms in our experiment. Three different silicate concentrations were set for cultivation, and <em>Skeletonema dohrnii</em> at the exponential growth phase was selected to analyze basic physiological parameters, polyamine content, and Polyamine oxidase (PAO) gene expression under varying silicate concentrations. Results showed that low silicate concentrations led to reduced growth rate and polyamine content, with down-regulation of PAO gene expression. Conversely, high silicate concentrations did not significantly increase growth rate but did increase polyamine content and up-regulate the PAO gene. These findings suggested that changes in silicate concentration impact the growth rate and polyamine content of <em>Skeletonema dohrnii</em>, with the PAO gene potentially playing a role in regulating the algal response to silicate concentration variations. This study provides a foundation for further exploration of the PAO gene function in <em>Skeletonema dohrnii</em>.</div></div>","PeriodicalId":18204,"journal":{"name":"Marine environmental research","volume":"204 ","pages":"Article 106860"},"PeriodicalIF":3.0000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of silicate on polyamine oxidase genes in Skeletonema dohrnii\",\"authors\":\"Wei Teng , Xiaomei Shang , Jun Sun\",\"doi\":\"10.1016/j.marenvres.2024.106860\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>By using <em>Skeletonema dohrnii</em> as the experimental algal species, we investigated how silicate concentration impacts the polyamine metabolism of diatoms in our experiment. Three different silicate concentrations were set for cultivation, and <em>Skeletonema dohrnii</em> at the exponential growth phase was selected to analyze basic physiological parameters, polyamine content, and Polyamine oxidase (PAO) gene expression under varying silicate concentrations. Results showed that low silicate concentrations led to reduced growth rate and polyamine content, with down-regulation of PAO gene expression. Conversely, high silicate concentrations did not significantly increase growth rate but did increase polyamine content and up-regulate the PAO gene. These findings suggested that changes in silicate concentration impact the growth rate and polyamine content of <em>Skeletonema dohrnii</em>, with the PAO gene potentially playing a role in regulating the algal response to silicate concentration variations. This study provides a foundation for further exploration of the PAO gene function in <em>Skeletonema dohrnii</em>.</div></div>\",\"PeriodicalId\":18204,\"journal\":{\"name\":\"Marine environmental research\",\"volume\":\"204 \",\"pages\":\"Article 106860\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Marine environmental research\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S014111362400521X\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine environmental research","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S014111362400521X","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
在实验中，我们以多毛藻（Skeletonema dohrnii）为实验藻种，研究了硅酸盐浓度对硅藻多胺代谢的影响。我们设定了三种不同的硅酸盐浓度进行培养，并选择了处于指数生长期的Skeletonema dohrnii，分析了不同硅酸盐浓度下的基本生理参数、多胺含量和多胺氧化酶（PAO）基因表达。结果表明，低硅酸盐浓度导致生长速度和多胺含量降低，PAO基因表达下调。相反，高浓度硅酸盐并未显著提高生长率，但却增加了多胺含量，并上调了 PAO 基因的表达。这些研究结果表明，硅酸盐浓度的变化会影响藻类的生长速率和多胺含量，而 PAO 基因可能在调节藻类对硅酸盐浓度变化的反应中发挥作用。这项研究为进一步探索多孔骨藻 PAO 基因的功能奠定了基础。
The effect of silicate on polyamine oxidase genes in Skeletonema dohrnii
By using Skeletonema dohrnii as the experimental algal species, we investigated how silicate concentration impacts the polyamine metabolism of diatoms in our experiment. Three different silicate concentrations were set for cultivation, and Skeletonema dohrnii at the exponential growth phase was selected to analyze basic physiological parameters, polyamine content, and Polyamine oxidase (PAO) gene expression under varying silicate concentrations. Results showed that low silicate concentrations led to reduced growth rate and polyamine content, with down-regulation of PAO gene expression. Conversely, high silicate concentrations did not significantly increase growth rate but did increase polyamine content and up-regulate the PAO gene. These findings suggested that changes in silicate concentration impact the growth rate and polyamine content of Skeletonema dohrnii, with the PAO gene potentially playing a role in regulating the algal response to silicate concentration variations. This study provides a foundation for further exploration of the PAO gene function in Skeletonema dohrnii.
期刊介绍：
Marine Environmental Research publishes original research papers on chemical, physical, and biological interactions in the oceans and coastal waters. The journal serves as a forum for new information on biology, chemistry, and toxicology and syntheses that advance understanding of marine environmental processes.
Submission of multidisciplinary studies is encouraged. Studies that utilize experimental approaches to clarify the roles of anthropogenic and natural causes of changes in marine ecosystems are especially welcome, as are those studies that represent new developments of a theoretical or conceptual aspect of marine science. All papers published in this journal are reviewed by qualified peers prior to acceptance and publication. Examples of topics considered to be appropriate for the journal include, but are not limited to, the following:
– The extent, persistence, and consequences of change and the recovery from such change in natural marine systems
– The biochemical, physiological, and ecological consequences of contaminants to marine organisms and ecosystems
– The biogeochemistry of naturally occurring and anthropogenic substances
– Models that describe and predict the above processes
– Monitoring studies, to the extent that their results provide new information on functional processes
– Methodological papers describing improved quantitative techniques for the marine sciences.
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