脐带间充质干细胞促进糖尿病根尖牙周炎动物模型骨再生

Eric Priyo Prasetyo , Pravinna Saravanan , Deaniddo Kharisna , Christina Immee Wijanarko , Mefina Kuntjoro , Nike Hendrijantini , Evelyn Tjendronegoro
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引用次数: 0

摘要

背景以往的研究显示糖尿病患者有持续性根尖牙周炎的倾向。通过牙髓治疗再生干细胞有望成为一种解决方案。本研究通过成骨细胞的组织病理学分析和runt相关转录因子2 (Runx2)和Osterix的免疫组化分析来评估糖尿病大鼠根尖牙周炎的骨再生。方法对20只大鼠进行糖尿病和根尖牙周炎诱导实验。麻醉下右下颌第一磨牙诱发根尖牙周炎。拔牙后开放7 d,进行根管治疗,随机分为4组(每组5只)。第一组和第二组分别于第30天(C30)和第60天(C60)结束,作为对照组。第三组和第四组给予脐带间充质干细胞,分别于30天(T30)和60天(T60)结束治疗。对成骨细胞、Runx2、Osterix进行分析。采用方差分析和Tukey HSD检验进行分析。p值的差异<;0.05为显著性。结果对照组(C30、C60)和治疗组(T30、T60)根尖区成骨细胞数量显著增加(p <;0.05)。对照组(C30和C60)和治疗组(T30和T60)成骨细胞中Runx2和Osterix的表达均显著升高(p <;0.05)。结论脐带间充质干细胞通过成骨细胞、Runx2和Osterix促进糖尿病根尖牙周炎动物模型的骨再生。
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Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis

Background

Previous studies revealed diabetes mellitus subjects tend to have persistent apical periodontitis. Regenerative stem cells therapy through endodontic procedure is hoped to be a solution. This study assessed bone regeneration in diabetic rats with apical periodontitis through histopathological analysis of osteoblasts and immunohistochemical analysis of runt-related transcription factor 2 (Runx2) and Osterix.

Methods

Diabetes mellitus and apical periodontitis was induced on 20 rats. Apical periodontitis was induced on mandibular right first molars under anesthesia. The teeth were left open for 7 days following access cavity and pulp extirpation, then the rats’ teeth were endodontically treated and randomly allocated into 4 groups (5 rats per group). The first and second groups was ended at 30 days (C30) and 60 days (C60) and labelled as control. The third and fourth groups was given umbilical cord mesenchymal stem cells and ended at 30 days (T30) and 60 days (T60). The osteoblasts, Runx2 and Osterix were analyzed. ANOVA and Tukey HSD tests were used for analysis. Differences with p values < 0.05 were considered significant.

Results

The number of osteoblasts in the apical area in control groups (C30 and C60) and treatment groups (T30 and T60) showed a significant increase (p < 0.05). The expressions of Runx2 and Osterix in osteoblasts showed a significant increase among the control (C30 and C60) and treatment groups (T30 and T60) (p < 0.05).

Conclusion

Umbilical cord mesenchymal stem cells improve bone regeneration in diabetic animal model with apical periodontitis, in terms of osteoblasts, Runx2 and Osterix.
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来源期刊
CiteScore
4.90
自引率
0.00%
发文量
133
审稿时长
167 days
期刊介绍: Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.
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