生酮饮食调节免疫细胞转录景观和改善实验性自身免疫性葡萄膜炎小鼠。

IF 9.3 1区 医学 Q1 IMMUNOLOGY Journal of Neuroinflammation Pub Date : 2024-12-03 DOI:10.1186/s12974-024-03308-z
Runping Duan, Tianfu Wang, Zhaohuai Li, Loujing Jiang, Xiaoyang Yu, Daquan He, Tianyu Tao, Xiuxing Liu, Zhaohao Huang, Lei Feng, Wenru Su
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引用次数: 0

摘要

背景:葡萄膜炎表现为眼内免疫介导的炎症性疾病,对视力构成严重威胁。生酮饮食(KD)已成为一种有前景的饮食干预,但其对免疫微环境的影响及其在葡萄膜炎中的作用尚不清楚。方法:利用小鼠淋巴结和视网膜的单细胞RNA测序(scRNA-seq)数据,全面研究KD对免疫微环境的影响。流式细胞术验证了潜在的机制。结果:本研究表明KD改变了免疫谱的组成和功能。具体而言,KD促进Treg细胞的分化,并提高其在健康小鼠中的比例。在应对实验性自身免疫性葡萄膜炎挑战时,KD可缓解炎症症状,降低CD4+ T细胞致病性,并纠正Th17/Treg失衡。此外,KD降低了Th17细胞的比例,增加了视网膜中Treg细胞的比例。对视网膜和ccdn联合免疫细胞的分析显示,视网膜免疫细胞,特别是CD4+ T细胞,表现出增强的炎症反应,KD部分逆转了这一反应。结论:KD诱导免疫细胞从淋巴结到视网膜的抑制性结构和功能改变,提示其治疗葡萄膜炎的潜力。
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Ketogenic diet modulates immune cell transcriptional landscape and ameliorates experimental autoimmune uveitis in mice.

Background: Uveitis manifests as immune-mediated inflammatory disorders within the eye, posing a serious threat to vision. The ketogenic diet (KD) has emerged as a promising dietary intervention, yet its impact on the immune microenvironments and role in uveitis remains unclear.

Methods: Utilizing single-cell RNA sequencing (scRNA-seq) data from lymph node and retina of mice, we conduct a comprehensive investigation into the effects of KD on immune microenvironments. Flow cytometry is conducted to verify the potential mechanisms.

Results: This study demonstrates that KD alters the composition and function of immune profiles. Specifically, KD promotes the differentiation of Treg cells and elevates its proportion in heathy mice. In response to experimental autoimmune uveitis challenges, KD alleviates the inflammatory symptoms, lowers CD4+ T cell pathogenicity, and corrects the Th17/Treg imbalance. Additionally, KD decreases the proportion of Th17 cell and increases Treg cells in the retina. Analysis of combined retinal and CDLN immune cells reveals that retinal immune cells, particularly CD4+ T cells, exhibit heightened inflammatory responses, which KD partially reverses.

Conclusions: The KD induces inhibitory structural and functional alterations in immune cells from lymph nodes to retina, suggesting its potential as a therapy for uveitis.

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来源期刊
Journal of Neuroinflammation
Journal of Neuroinflammation 医学-神经科学
CiteScore
15.90
自引率
3.20%
发文量
276
审稿时长
1 months
期刊介绍: The Journal of Neuroinflammation is a peer-reviewed, open access publication that emphasizes the interaction between the immune system, particularly the innate immune system, and the nervous system. It covers various aspects, including the involvement of CNS immune mediators like microglia and astrocytes, the cytokines and chemokines they produce, and the influence of peripheral neuro-immune interactions, T cells, monocytes, complement proteins, acute phase proteins, oxidative injury, and related molecular processes. Neuroinflammation is a rapidly expanding field that has significantly enhanced our knowledge of chronic neurological diseases. It attracts researchers from diverse disciplines such as pathology, biochemistry, molecular biology, genetics, clinical medicine, and epidemiology. Substantial contributions to this field have been made through studies involving populations, patients, postmortem tissues, animal models, and in vitro systems. The Journal of Neuroinflammation consolidates research that centers around common pathogenic processes. It serves as a platform for integrative reviews and commentaries in this field.
期刊最新文献
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