panaxadiol抗帕金森病作用机制及制备研究。

IF 6.7 1区 医学 Q1 CHEMISTRY, MEDICINAL Phytomedicine Pub Date : 2025-01-01 DOI:10.1016/j.phymed.2024.156296
Lei Xu , Zhongjin Zhang , Ziqi Feng , Sixiang Niu , Lixia Yang , Baoguo Xiao , Xiao-ming Jin , Cungen Ma , Huijie Fan , Shengnan Xiao , Zhi Chai
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引用次数: 0

摘要

背景:中医为探索疾病治疗的新途径提供了独特而宝贵的资源。人参二醇,一种由人参皂苷转化而来的活性化合物。它具有多种药理活性。然而,关于其对帕金森病(PD)的影响,缺乏研究。目的与研究设计:本研究从体内和体外两方面探讨了panaxadiol治疗PD的活性和作用机制。此外,还建立了一种提取panaxadiol的新方法。结果:结果表明,在体外,panaxadiol可以降低LPS诱导的BV2细胞的活力和细胞毒性,并通过TLR4/MyD88/NF-κB途径抑制促炎因子的产生。在体内,panaxadiol可以改善PD小鼠的运动和肠道功能障碍,修复DA神经元的缺失。进一步的研究表明,panaxadiol治疗可以减轻血脑屏障(BBB)的损伤,抑制黑质(SN)的神经炎症,并进一步减轻DA神经元的损伤。随后发现,panaxadiol可以减轻外周炎症,并显著恢复肠道微生物群落。进一步的机制研究发现,panaxadiol治疗可抑制SN中TLR4/MyD88/NF-κB信号通路及其下游促炎产物。此外,利用响应面法确定了深层共晶溶剂(NADES)提取panaxadiol的最佳条件。结论:综上所述,本研究首次发现,panaxadiol可通过调节神经炎症、修复血脑屏障和肠道微生物群,有效改善PD的症状。同时,采用绿色提取工艺显著提高了panaxadiol的含量,为PD候选药物的开发提供了新的方向。
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Study on the anti-Parkinson's disease activity mechanism and preparation of panaxadiol

Background

Traditional Chinese medicine offers unique and valuable resources for the exploration of novel approaches to disease treatment. Panaxadiol, an active compound derived from the transformation of ginsenosides. It has a variety of pharmacological activities. However, there is a lack of research regarding its effects on Parkinson's disease (PD).

Purpose and Study design

This study investigates the activity and mechanism of panaxadiol in the treatment of PD both in vivo and in vitro. In addition, a new method has been developed to extract panaxadiol.

Results

The results showed that in vitro, panaxadiol can reduce the viability and cytotoxicity of BV2 cells induced by LPS, and inhibit the production of pro-inflammatory factors through the TLR4/MyD88/NF-κB pathway. In vivo, panaxadiol can improve motor and intestinal dysfunction in PD mice and repair the loss of DA neurons. Further studies have shown that panaxadiol treatment alleviates damage to the blood-brain barrier (BBB), inhibits neuroinflammation in the substantia nigra (SN), and further reduces damage to DA neurons. Subsequently, it was found that panaxadiol alleviated peripheral inflammation and significantly restored the intestinal microbial community. Further mechanistic studies found that panaxadiol treatment inhibited the TLR4/MyD88/NF-κB signaling pathway and its downstream pro-inflammatory products in the SN. In addition, Additionally, the response surface method was employed to identify the optimal conditions for extracting panaxadiol using a deep eutectic solvent (NADES).

Conclusion

In summary, this study has found for the first time that panaxadiol can effectively improve the symptoms of PD by regulating neuroinflammation, repairing the BBB, and gut microbiota. Meanwhile, adopting a green extraction process significantly increases the content of panaxadiol, providing a new direction for the development of PD candidate drugs.
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来源期刊
Phytomedicine
Phytomedicine 医学-药学
CiteScore
10.30
自引率
5.10%
发文量
670
审稿时长
91 days
期刊介绍: Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.
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