理化因素对噬菌体RB49质粒转导频率影响的研究

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-12-20 DOI:10.1134/S0006350924700532
A. N. Nikulina, N. A. Nikulin, A. A. Zimin
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引用次数: 0

摘要

与 T4 相关的噬菌体 RB49 能够以相对较高的频率进行一般质粒转导。由于这种机制,细菌可以获得适应不断变化的环境条件和探索新生态位的能力。我们研究了 pH 值、温度和长波紫外线(λ = 366 nm)照射对噬菌体 RB49 制剂特性的影响,该制剂包含带有 pTurboGFP-B 质粒 DNA 的转导颗粒和带有自身 DNA 的毒性颗粒。研究获得了毒粒滴度变化和 RB49 噬菌体转导 pTurboGFP-B 质粒频率的数据。紫外线照射 2 小时后,RB49 噬菌体转导 pTurboGFP-B 质粒的频率增加了约 3 倍。在冰中保存 40 分钟后,转导频率增加了数倍。根据我们获得的实验数据推测,RB49噬菌体的转导颗粒可能比毒性颗粒更能抵抗长波紫外线辐射和接近 0°C 的温度,并能比正常条件下更有效地完成转导过程。在光照充足的水库(包括寒冷的水库)中也可能发生类似的过程,其中可能存在与 RB49 相关的噬菌体。这表明,水生生态环境中的横向基因转移可能比以前假设的更为密集。
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A Study of the Effects of Physico-Chemical Factors on the Frequency of Plasmid Transduction by Bacteriophage RB49

T4-related bacteriophage RB49 is capable of general plasmid transduction with a relatively high frequency. Due to this mechanism, bacteria can acquire the ability to adapt to changing environmental conditions and explore new ecological niches. The effects of pH, temperature, and irradiation with long-wavelength ultraviolet light (λ = 366 nm) on the characteristics of the phage RB49 preparation containing transducing particles with pTurboGFP-B plasmid DNA and virulent particles with their own DNA were studied. Data on changes in the titer of virulent particles and the frequency of transduction of the pTurboGFP-B plasmid by the RB49 phage were obtained. After exposure to UV radiation for 2 h, the frequency of transduction of the pTurboGFP-B plasmid by the RB49 phage increased by ~3 times. After storing the preparation in ice for 40 min, a several-fold increase in the transduction frequency was observed. Based on the experimental data we obtained, it was assumed that the transducing particles of the RB49 phage may be more resistant to long-wavelength UV radiation and temperatures close to 0°C than virulent ones and provide the transduction process more efficiently than under normal conditions. Similar processes can occur in well-lit reservoirs, including cold ones, in which phages related to RB49 may occur. This indicates the possibility of more intensive horizontal gene transfer in aquatic ecotopes than previously assumed.

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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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