DCA基因过表达对转基因小杜氏藻的影响

Lingru Ruan, Bingfeng Zhou, Yanyan Liang, Xiumin Zhang, Lina Wu, Limei Huang, Shiyu Liang, Changhua Shang
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引用次数: 0

摘要

重复碳酸酐酶是碳酸酐酶家族的一员。普遍存在的碳酸酐酶(CA)在植物中起着重要的作用。然而,CA在微小杜氏藻中的作用仍有待进一步探讨。本研究从疟原虫中分离到CAs家族的一个成员,命名为DpDCA。通过基因工程技术将其转移到疟原虫中。类胡萝卜素、总碳水化合物、淀粉、蛋白质和油脂含量分别比对照提高了16.31%、31.68%、43.97%、52.91%和12.32%。可能DpDCA通过顺式元素影响光合作用。因此,本研究为研究DpDCA基因在d.p arva中的功能奠定了基础。
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Effects of DCA gene overexpression in transgenic Dunaliella parva

Duplicated carbonic anhydrase was a member of carbonic anhydrase family. The ubiquitous carbonic anhydrase (CA) plays important roles in plants. However, the role of CA in Dunaliella parva remains to be further explored. In this study, a member of the CAs family was isolated from D. parva and named DpDCA. It was transferred into D. parva by genetic engineering techniques. The carotenoid, total carbohydrate, starch, protein, and oil contents of transgenic D. parva increased by 16.31%, 31.68%, 43.97%, 52.91%, and 12.32%, compared to control. Perhaps DpDCA affected photosynthesis through cis-element. Therefore, this study lays a foundation for studying the function of DpDCA gene in D. parva.

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
期刊最新文献
Correction: Chengkuizengella axinellae sp. nov., a symbiotic bacterium isolated from a marine sponge of the genus Axinella Comprehensive genomic and phenotypic characterization of thermophilic bacterium Sinimarinibacterium thermocellulolyticum sp. nov. HSW-8T, a cellulase-producing bacterium isolated from hot spring water in South Korea Characterisation of Aequorivita ciconiae sp. nov., isolated from oriental stork, Ciconia boyciana Effects of DCA gene overexpression in transgenic Dunaliella parva Taxonomic and genomic characterization of a siderophore-producing bacterium, Rhodoligotrophos ferricapiens sp. nov isolated from lettuce cultivation soil
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