{"title":"PsHB7/12基因通过ABA的负反馈调控参与了新疆梨的越冬过程。","authors":"Xiaoyan Liu, Aowei Li, Xueying Yang, Guanghong Luo, Jianbo Zhu","doi":"10.1016/j.plaphy.2024.109440","DOIUrl":null,"url":null,"abstract":"<p><p>Pyrus sinkiangensis, a crucial economic fruit tree in Xinjiang, China, experiences winter hardiness that significantly influences its yield and fruit quality. This study aimed to investigate the role of PsHB7/12 in cold resistance of Pyrus sinkiangensis and its regulation of abscisic acid (ABA) signaling. Through physiological assessments and transcriptome analysis, we identified a peak expression of PsHB7/12 in January, which was strongly induced by ABA. We found a correlation between ABA concentrations and changes in water content and soluble protein levels during overwintering process. Further analysis of yeast one-hybrid and the luciferase assay revealed that PsHB7/12 was involved in the negative regulation of ABA signal by inhibiting the expression of PsPYL4. Additionally, the overexpression of the PsHB7/12 may have complex effects on ABA signaling through the modulation of expression of members of the PsPP2Cs family. In summary, PsHB7/12 regulates the ABA signaling pathway through a negative feedback mechanism. These findings reveal the critical role of PsHB7/12 in cold stress adaptation in Pyrus sinkiangensis and provide new molecular markers for breeding stress-resistant fruit trees.</p>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"220 ","pages":"109440"},"PeriodicalIF":6.1000,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PsHB7/12 gene participated in the overwintering process of Pyrus sinkiangensis through negative feedback regulation of ABA.\",\"authors\":\"Xiaoyan Liu, Aowei Li, Xueying Yang, Guanghong Luo, Jianbo Zhu\",\"doi\":\"10.1016/j.plaphy.2024.109440\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Pyrus sinkiangensis, a crucial economic fruit tree in Xinjiang, China, experiences winter hardiness that significantly influences its yield and fruit quality. This study aimed to investigate the role of PsHB7/12 in cold resistance of Pyrus sinkiangensis and its regulation of abscisic acid (ABA) signaling. Through physiological assessments and transcriptome analysis, we identified a peak expression of PsHB7/12 in January, which was strongly induced by ABA. We found a correlation between ABA concentrations and changes in water content and soluble protein levels during overwintering process. Further analysis of yeast one-hybrid and the luciferase assay revealed that PsHB7/12 was involved in the negative regulation of ABA signal by inhibiting the expression of PsPYL4. Additionally, the overexpression of the PsHB7/12 may have complex effects on ABA signaling through the modulation of expression of members of the PsPP2Cs family. In summary, PsHB7/12 regulates the ABA signaling pathway through a negative feedback mechanism. These findings reveal the critical role of PsHB7/12 in cold stress adaptation in Pyrus sinkiangensis and provide new molecular markers for breeding stress-resistant fruit trees.</p>\",\"PeriodicalId\":20234,\"journal\":{\"name\":\"Plant Physiology and Biochemistry\",\"volume\":\"220 \",\"pages\":\"109440\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-12-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Physiology and Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.plaphy.2024.109440\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.plaphy.2024.109440","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
PsHB7/12 gene participated in the overwintering process of Pyrus sinkiangensis through negative feedback regulation of ABA.
Pyrus sinkiangensis, a crucial economic fruit tree in Xinjiang, China, experiences winter hardiness that significantly influences its yield and fruit quality. This study aimed to investigate the role of PsHB7/12 in cold resistance of Pyrus sinkiangensis and its regulation of abscisic acid (ABA) signaling. Through physiological assessments and transcriptome analysis, we identified a peak expression of PsHB7/12 in January, which was strongly induced by ABA. We found a correlation between ABA concentrations and changes in water content and soluble protein levels during overwintering process. Further analysis of yeast one-hybrid and the luciferase assay revealed that PsHB7/12 was involved in the negative regulation of ABA signal by inhibiting the expression of PsPYL4. Additionally, the overexpression of the PsHB7/12 may have complex effects on ABA signaling through the modulation of expression of members of the PsPP2Cs family. In summary, PsHB7/12 regulates the ABA signaling pathway through a negative feedback mechanism. These findings reveal the critical role of PsHB7/12 in cold stress adaptation in Pyrus sinkiangensis and provide new molecular markers for breeding stress-resistant fruit trees.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.