囊性包虫病水牛肝脏的组织学、组织化学和免疫组织化学变化。

IF 2.3 2区 农林科学 Q2 PATHOLOGY Veterinary Pathology Pub Date : 2025-01-06 DOI:10.1177/03009858241309399
Humera Aslam, Hana Hussein M S Wardah, Kafil Akhtar, Sayeedul Hasan Arif, Malik Irshadullah
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引用次数: 0

摘要

本研究旨在评价囊性包虫病水牛肝脏的组织学、组织化学和免疫组织化学变化。从Aligarh屠宰场新屠宰的水牛身上收集了未感染和感染的肝脏。将感染和未感染的小块肝脏(n = 5)切开并处理以进行组织学和组织化学研究。采用兔抗cd3、CD19和CD117抗体进行免疫组化。结果显示,卵囊、生发膜和层状膜被纤维外包膜包围,其次是嗜酸性粒细胞、单核细胞、中性粒细胞、淋巴细胞的中度和弥漫性浸润,肥大细胞的局灶性浸润明显。感染的肝脏也有轻微的中央静脉和窦状静脉扩张,肝组织轻度和局灶性坏死,中央静脉和门静脉充血。周期性的酸-希夫反应显示感染肝脏明显的糖原耗竭。Masson三色染色显示,与未感染的肝脏相比,门静脉区、外皮层和肝细胞之间有明显的胶原纤维沉积,未感染的肝脏仅在门静脉区发现沉积。在受感染的肝脏中,t细胞的反应比b细胞的反应更明显。由此可见,包虫病感染可引起多种病理生化变化,感染肝脏炎症细胞浸润增加,提示包虫病涉及非特异性免疫应答。t细胞反应比b细胞更明显,表明细胞介导的免疫参与了对囊性包虫病的免疫。这些发现可能有助于了解囊性包虫病的局部免疫反应。
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Histologic, histochemical, and immunohistochemical changes in buffalo liver with cystic echinococcosis.

The present study aimed to evaluate the histologic, histochemical, and immunohistochemical changes in buffalo livers with cystic echinococcosis. Noninfected and infected livers were collected from the freshly slaughtered buffalo at the Aligarh abattoir. Small pieces of both infected and noninfected livers (n = 5) were cut and processed for histologic and histochemical studies. Immunohistochemistry was performed using rabbit anti-CD3, CD19, and CD117 antibodies. The results revealed the presence of brood capsules and germinal and laminated membranes surrounded by a fibrous adventitial layer, followed by moderate and diffused infiltration of eosinophils, monocytes, neutrophils, lymphocytes, and marked focal infiltration of mast cells. The infected livers also had mild dilation of central veins and sinusoids, mild and focal necrosis of hepatic tissue, and congestion of central and portal veins. Periodic acid-Schiff reaction revealed marked glycogen depletion in the infected liver. Masson's trichrome stain showed marked deposition of collagen fibers in the portal area, adventitial layer, and between the hepatocytes compared with the noninfected liver, where deposition was found only in the portal area. The T-cell response was more pronounced than the B-cell response in infected liver. Thus, it can be concluded that hydatid cyst infection causes several pathological and biochemical changes and increased infiltration of inflammatory cells in the infected livers, suggesting the involvement of nonspecific immune responses against hydatid cysts. The T-cell response was more pronounced than B-cells, indicating the involvement of cell-mediated immunity against cystic echinococcosis. These findings may help to understand the local immune responses to cystic echinococcosis.

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来源期刊
Veterinary Pathology
Veterinary Pathology 农林科学-病理学
CiteScore
4.70
自引率
8.30%
发文量
99
审稿时长
2 months
期刊介绍: Veterinary Pathology (VET) is the premier international publication of basic and applied research involving domestic, laboratory, wildlife, marine and zoo animals, and poultry. Bridging the divide between natural and experimental diseases, the journal details the diagnostic investigations of diseases of animals; reports experimental studies on mechanisms of specific processes; provides unique insights into animal models of human disease; and presents studies on environmental and pharmaceutical hazards.
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