Serum metabolic fingerprinting on Ag@AuNWs for traumatic brain injury diagnosis.

Accurate and rapid diagnosis of traumatic brain injury (TBI) is essential for high-quality medical services. Nonetheless, the current diagnostic platform still has challenges in rapidly and accurately analysing clinical samples. Here, we prepared a highly stable, repeatable and sensitive gold-plated silver core-shell nanowire (Ag@AuNWs) for surface-enhanced Raman spectroscopy (SERS) metabolic fingerprint diagnosis of TBI. The core-shell structure significantly enhanced SERS intensity and enables the direct detection of 10 μL serum within seconds. The principal component analysis-linear discriminant analysis (PCA-LDA) and partial least squaresdiscriminant analysis (PLS-DA) are used to evaluate the classification effect of this technology on TBI, respectively. The diagnosis accuracy rate of PCA-LDA and PLS-DA is 73.3% and 86.7% for diagnosing TBI, respectively. Consequently, the PLS-DA model is the optimal selection for distinguishing between the TBI and sham groups. This research will facilitate the application-oriented creation of novel materials with tailored structural designs and the formulation of innovative precision medical protocols in the imminent future. .