CCD4(4B)的差异表达驱动草莓果实类胡萝卜素含量的自然变异。

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Journal Pub Date : 2025-01-22 DOI:10.1111/pbi.14523
Iraida Amaya, F. Javier Roldán-Guerra, José L. Ordóñez-Díaz, Rocío Torreblanca, Henning Wagner, Veronika Waurich, Klaus Olbricht, José M. Moreno-Rojas, José F. Sánchez-Sevilla, Cristina Castillejo
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引用次数: 0

摘要

类胡萝卜素是一组多样的色素,赋予许多园艺植物红色、橙色和黄色的色调,也增强了它们的营养特性和健康益处。在草莓中,调节水果类胡萝卜素组成自然变化的遗传和分子机制仍未得到充分研究。在本研究中,我们使用黄/白肉群体分离检测了一个主要的数量性状位点(QTL), qYellow flesh -4B,位于4B染色体上,占总表型变异的82%。在QTL区间,类胡萝卜素裂解双加氧酶CCD4(4B)启动子的特异性多态性与果肉变黄、成熟过程中CCD4(4B)下调和类胡萝卜素含量增加相关。CCD4(4B)在草莓果实中的瞬时过表达作用进一步证实了CCD4(4B)在类胡萝卜素转化中的作用,导致黄叶素、紫黄质、叶黄素和玉米黄质浓度降低。值得注意的是,CCD4(4B)启动子中的- 35 C>;T单核苷酸多态性(SNP)可以预测不同八倍体Fragaria物种的CCD4(4B)表达和类胡萝卜素含量。这些发现为草莓中类胡萝卜素组成和积累的自然变异提供了有价值的遗传见解。本研究开发的高分辨率融化(HRM) DNA测试为预测草莓果实中高类胡萝卜素含量提供了一种快速可靠的方法,为旨在提高该作物营养价值的育种项目提供了有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Differential expression of CCD4(4B) drives natural variation in fruit carotenoid content in strawberry (Fragaria spp.)

Carotenoids are a diverse group of pigments imparting red, orange, and yellow hues to many horticultural plants, also enhancing their nutritional properties and health benefits. In strawberry, the genetic and molecular mechanisms regulating the natural variation of fruit carotenoid composition remain largely unexplored. In this study, we use a population segregating in yellow/white flesh to detect a major quantitative trait locus (QTL), qYellow Flesh-4B, located on chromosome 4B and accounting for 82% of total phenotypic variation. In the QTL interval, specific polymorphisms on the promoter of the carotenoid cleavage dioxygenase CCD4(4B) were associated with yellow flesh, down-regulation of CCD4(4B) during ripening, and increased carotenoid content. The role of CCD4(4B) in carotenoid turnover was further confirmed through transient overexpression in strawberry fruits, which resulted in decreased concentrations of the xanthophylls violaxanthin, lutein, and zeaxanthin. Notably, a −35 C>T single-nucleotide polymorphism (SNP) in the CCD4(4B) promoter was predictive of both CCD4(4B) expression and carotenoid content across a diverse collection of octoploid Fragaria species. These findings provide valuable genetic insights into the natural variation of carotenoid composition and accumulation in strawberry. A high-resolution melting (HRM) DNA test developed in this study offers a rapid and reliable method for predicting high carotenoid content in strawberry fruits, representing a valuable tool for breeding projects aimed at enhancing the nutritional value of this crop.

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来源期刊
Plant Biotechnology Journal
Plant Biotechnology Journal 生物-生物工程与应用微生物
CiteScore
20.50
自引率
2.90%
发文量
201
审稿时长
1 months
期刊介绍: Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.
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