Promising mass spectrometry imaging: exploring microscale insights in food.

This review focused on mass spectrometry imaging (MSI), a powerful tool in food analysis, covering its ion source schemes and procedures and their applications in food quality, safety, and nutrition to provide detailed insights into these aspects. The review presented a detailed introduction to both commonly used and emerging ionization sources, including nanoparticle laser desorption/ionization (NPs-LDI), air flow-assisted ionization (AFAI), desorption ionization with through-hole alumina membrane (DIUTHAME), plasma-assisted laser desorption ionization (PALDI), and low-temperature plasma (LTP). In the MSI process, particular emphasis was placed on quantitative MSI (QMSI) and super-resolution algorithms. These two aspects synergistically enhanced MSI's analytical capabilities: QMSI enabled accurate relative and absolute quantification, providing reliable data for composition analysis, while super-resolution algorithms improved molecular spatial imaging resolution, facilitating biomarker and trace substance detection. MSI outperformed conventional methods in comprehensively exploring food functional factors, biomarker discovery, and monitoring processing/storage effects by discerning molecular species and their spatial distributions. However, challenges such as immature techniques, complex data processing, non-standardized instruments, and high costs existed. Future trends in instrument enhancement, multispectral integration, and data analysis improvement were expected to deepen our understanding of food chemistry and safety, highlighting MSI's revolutionary potential in food analysis and research.