Linna Gao , Haiquan Ding , Qianqian Shi , Xinxin Wang, Leying Zhang, Ling Yang
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Pregnancy-induced changes in the toll-like receptor pathway in the ovine duodenum
Toll-like receptor (TLR) signaling plays a key role in the intestinal innate and adaptive immune responses, and pregnancy has effects on intestinal length and villus height. Nevertheless, it is unclear if early pregnancy modulates the expression of TLR members in the duodenum of ewes. In this study, ovine duodenums were obtained at day 16 of the estrous cycle (N16), and days 13, 16, and 25 of gestation (P13, P16, and P25) from ewes, and mRNA and protein expression of TLR members were analyzed. The results showed that early pregnancy increased the expression of TLR3, TLR5, and TRAF6, but did not influence TLR4 expression. In addition, there were increases in expression of TLR2, MyD88, and IRAK1 at P13 and P16, or only at P16 compared to N16 and P25. In summary, these data suggest that early pregnancy modulates the TLR signal pathway in the maternal duodenum, which may be related to the immunoregulation and adaptation of the maternal duodenum in the ovine.
期刊介绍：
The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease.
Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above.
The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.
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