一种新的直接检测葡萄凝固酶的显色法

Małgorzata Bulanda , Zenon Weęgrzynowicz , Marek Ciurak , Janusz Kowalczyk , Gotfryd Kupryszewski , Maria Gruszka , Piotr B. Heczko , Gerhard Pulverer
{"title":"一种新的直接检测葡萄凝固酶的显色法","authors":"Małgorzata Bulanda ,&nbsp;Zenon Weęgrzynowicz ,&nbsp;Marek Ciurak ,&nbsp;Janusz Kowalczyk ,&nbsp;Gotfryd Kupryszewski ,&nbsp;Maria Gruszka ,&nbsp;Piotr B. Heczko ,&nbsp;Gerhard Pulverer","doi":"10.1016/S0176-6724(88)80147-0","DOIUrl":null,"url":null,"abstract":"<div><p>Tube test for detection of staphylococcal coagulase, despite of many disadvantages, is commonly used in clinical microbiology for identification of <em>Staphylococcus aureus</em>. In this paper a new chromogenic method for detection of the coagulase directly in staphylococcal cultures is described and evaluated on the basis of a comparison with the standard tube assay. The chromogenic assay appeared to be as sensitive as the tube test but results of the former one can be read in a few hours without any apparatus.</p></div><div><p>Trotz mancher Nachteile wird der Röhrchentest generell zum Nachweis der Staphylokokken-Koagulase in der medizinischen Mikrobiologie verwendet, um die Spezies <em>Staphylococcus aureus</em> zu identifizieren. In vorliegender Arbeit wird eine neue chromogene Methode zum Nachweis der Koagulase direkt in der Staphylokokken-Kultur vorgestellt und mit dem Standard-Röhrchentest verglichen. Der chromogene Nachweistest erwies sich dem Standard-Röhrchentest als ebenbürtig, kann aber in wenigen Stunden ohne apparative Hilfe abgelesen werden.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 115-121"},"PeriodicalIF":0.0000,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80147-0","citationCount":"5","resultStr":"{\"title\":\"A New Chromogenic Assay for Direct Detection of Staphylocoagulase\",\"authors\":\"Małgorzata Bulanda ,&nbsp;Zenon Weęgrzynowicz ,&nbsp;Marek Ciurak ,&nbsp;Janusz Kowalczyk ,&nbsp;Gotfryd Kupryszewski ,&nbsp;Maria Gruszka ,&nbsp;Piotr B. Heczko ,&nbsp;Gerhard Pulverer\",\"doi\":\"10.1016/S0176-6724(88)80147-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Tube test for detection of staphylococcal coagulase, despite of many disadvantages, is commonly used in clinical microbiology for identification of <em>Staphylococcus aureus</em>. In this paper a new chromogenic method for detection of the coagulase directly in staphylococcal cultures is described and evaluated on the basis of a comparison with the standard tube assay. The chromogenic assay appeared to be as sensitive as the tube test but results of the former one can be read in a few hours without any apparatus.</p></div><div><p>Trotz mancher Nachteile wird der Röhrchentest generell zum Nachweis der Staphylokokken-Koagulase in der medizinischen Mikrobiologie verwendet, um die Spezies <em>Staphylococcus aureus</em> zu identifizieren. In vorliegender Arbeit wird eine neue chromogene Methode zum Nachweis der Koagulase direkt in der Staphylokokken-Kultur vorgestellt und mit dem Standard-Röhrchentest verglichen. Der chromogene Nachweistest erwies sich dem Standard-Röhrchentest als ebenbürtig, kann aber in wenigen Stunden ohne apparative Hilfe abgelesen werden.</p></div>\",\"PeriodicalId\":101291,\"journal\":{\"name\":\"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology\",\"volume\":\"270 1\",\"pages\":\"Pages 115-121\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80147-0\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0176672488801470\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176672488801470","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

摘要

检测葡萄球菌的检测,阴性分流的大白天通用财务联合报用于查明葡萄球菌。在这幅新的鞋中,有一种全新的寻找行为的直觉的方法,就是在标准审计的基础上进行评价。那种让人对音乐敏感的次品只是因为尽管有一些缺点,但一般而言,管状动脉检查常用来证明医疗微生物学中有一种葡萄球菌凝固剂,从而识别这种葡萄球菌。介绍了一种新的次品次品测试为标准套筒次品
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
A New Chromogenic Assay for Direct Detection of Staphylocoagulase

Tube test for detection of staphylococcal coagulase, despite of many disadvantages, is commonly used in clinical microbiology for identification of Staphylococcus aureus. In this paper a new chromogenic method for detection of the coagulase directly in staphylococcal cultures is described and evaluated on the basis of a comparison with the standard tube assay. The chromogenic assay appeared to be as sensitive as the tube test but results of the former one can be read in a few hours without any apparatus.

Trotz mancher Nachteile wird der Röhrchentest generell zum Nachweis der Staphylokokken-Koagulase in der medizinischen Mikrobiologie verwendet, um die Spezies Staphylococcus aureus zu identifizieren. In vorliegender Arbeit wird eine neue chromogene Methode zum Nachweis der Koagulase direkt in der Staphylokokken-Kultur vorgestellt und mit dem Standard-Röhrchentest verglichen. Der chromogene Nachweistest erwies sich dem Standard-Röhrchentest als ebenbürtig, kann aber in wenigen Stunden ohne apparative Hilfe abgelesen werden.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
The Bactec-system in the diagnosis of tuberculosis comparison of a conventional and the radiometric method (Bactec) for culturing, differentiation and susceptibility testing of Mycobacteria GasPak plus versus anaerocult a — two carbon dioxide/hydrogen systems for cultivation of anaerobes Monoclonal antibodies to protein I for serotyping of Neisseria gonorrhoeae: Correlation of serotype with bactericidal activity Genome type analysis of certain adenovirus serotypes of subgenus D Detection and interspecies-transformation of a β-Lactamase-encoding plasmid from Pasteurella haemolytica
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1