被碳氢化合物污染的地下水的微生物学研究。1. 细菌种群的定量和定性分布。

W Dott, C Frank, P Werner
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引用次数: 0

摘要

受碳氢化合物污染的地下水在一年的检测中显示出大量的细胞数:在20℃条件下,n -琼脂在5天后生长10(3)-10(4)CFU/ml,在20℃条件下,8周在水琼脂上生长10(4)-10(5)CFU/ml,总细胞数为10(5)-10(7)/ml。从n -琼脂中分离到的细菌主要为假单胞菌、黄杆菌、不动杆菌等革兰氏阴性菌,革兰氏阳性菌属有节杆菌和芽孢杆菌。此外,在地下水中还发现了以下“生理”类群:氨氧化细菌(MPN 10(1)-10(2)/ml)、硫氧化细菌(MPN 10(1)-10(4)/ml)、硝酸盐还原细菌(MPN 10(3)-10(6)/ml)、反硝化细菌(MPN 10(1)-10(4)/ml)、需氧孢子形成细菌孢子(2-30/ml)、厌氧孢子形成细菌孢子(0.2-0.4/ml)、锰氧化细菌(10(3)-10(4)CFU/ml)和放线菌(0.1-1.0 CFU/ml)。
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[Microbiological studies of ground water polluted with hydrocarbons. 1. Quantitative and qualitative distribution of bacterial populations].

The groundwater, polluted with hydrocarbons, showed large cell numbers during one year of examination: 10(3)-10(4) CFU/ml grown on N-agar after 5 days at 20 degrees C, 10(4)-10(5) CFU/ml grown on water-agar after 8 weeks at 20 degrees C and total cell numbers of 10(5)-10(7)/ml. The bacteria, which were isolated from N-agar, were chiefly Pseudomonas, Flavobacterium, Acinetobacter, and other gramnegative species, Arthrobacter and Bacillus as members of grampositive bacteria. Furthermore the following "physiological' groups were found in the groundwater: ammonium-oxidizing bacteria (MPN 10(1)-10(2)/ml), sulfur-oxidizing bacteria (MPN 10(1)-10(4)/ml), nitrate-reducing bacteria (MPN 10(3)-10(6)/ml), denitrifying bacteria (MPN 10(1)-10(4)/ml), spores of aerobe spore-forming bacteria (2-30/ml), spores of anaerobe spore-forming bacteria (0.2-0.4/ml), manganese-oxidizing bacteria (10(3)-10(4) CFU/ml) and Actinomycetes (0.1-1.0 CFU/ml).

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