{"title":"用随机扩增的多态DNA鉴定绿虫(同翅目:蚜虫科)生物型","authors":"Felix O Aikhionbare , Kenneth P Pruess , Z.B Mayo","doi":"10.1016/S1050-3862(98)00002-3","DOIUrl":null,"url":null,"abstract":"<div><p>Genomic DNA was extracted from seven greenbug, <em>Schizaphis graminum</em>, biotypes (B, C, E, F, G, H and I) obtained from laboratory colonies maintained by USDA-ARS, Stillwater, Oklahoma. DNA was amplified using single 10-base primers. Of 100 primers tested, four were found which either alone, or in combination, distinguished all biotypes by distinct size differences in amplified fragments. Results were repeatable using aphids obtained from the same colonies 2 years later. These diagnostic primers produced unvarying banding patterns for all biotype E greenbugs collected in the field in Nebraska, Kansas, Oklahoma, and Texas.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 4","pages":"Pages 105-108"},"PeriodicalIF":0.0000,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00002-3","citationCount":"9","resultStr":"{\"title\":\"Greenbug (Homoptera: Aphididae) biotypes characterized using random amplified polymorphic DNA\",\"authors\":\"Felix O Aikhionbare , Kenneth P Pruess , Z.B Mayo\",\"doi\":\"10.1016/S1050-3862(98)00002-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Genomic DNA was extracted from seven greenbug, <em>Schizaphis graminum</em>, biotypes (B, C, E, F, G, H and I) obtained from laboratory colonies maintained by USDA-ARS, Stillwater, Oklahoma. DNA was amplified using single 10-base primers. Of 100 primers tested, four were found which either alone, or in combination, distinguished all biotypes by distinct size differences in amplified fragments. Results were repeatable using aphids obtained from the same colonies 2 years later. These diagnostic primers produced unvarying banding patterns for all biotype E greenbugs collected in the field in Nebraska, Kansas, Oklahoma, and Texas.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"14 4\",\"pages\":\"Pages 105-108\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00002-3\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386298000023\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386298000023","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
摘要
从美国农业部农业研究中心(USDA-ARS, Stillwater, Oklahoma)维护的实验室菌落中获得的7种绿虫(Schizaphis graminum, B, C, E, F, G, H和I)生物型中提取基因组DNA。使用单个10碱基引物扩增DNA。在测试的100个引物中，发现有4个引物单独或组合，通过扩增片段的明显大小差异来区分所有生物型。2年后使用同一菌落获得的蚜虫，结果可重复。这些诊断引物对内布拉斯加州、堪萨斯州、俄克拉荷马州和德克萨斯州收集的所有E型绿虫产生不变的条带模式。
Greenbug (Homoptera: Aphididae) biotypes characterized using random amplified polymorphic DNA
Genomic DNA was extracted from seven greenbug, Schizaphis graminum, biotypes (B, C, E, F, G, H and I) obtained from laboratory colonies maintained by USDA-ARS, Stillwater, Oklahoma. DNA was amplified using single 10-base primers. Of 100 primers tested, four were found which either alone, or in combination, distinguished all biotypes by distinct size differences in amplified fragments. Results were repeatable using aphids obtained from the same colonies 2 years later. These diagnostic primers produced unvarying banding patterns for all biotype E greenbugs collected in the field in Nebraska, Kansas, Oklahoma, and Texas.
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