日本幽门螺杆菌中空泡毒素vacA基因的变异。

Microbios Pub Date : 1999-01-01
T Ueda, M Kawaguchi, T Saito, M Ishigaki, H Hamashima, M Sasatsu, T Arai
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引用次数: 0

摘要

在日本分离到的284株幽门螺杆菌均经聚合酶链反应(PCR)证实为vacA基因阳性。PCR扩增得到的vacA基因分为6类(临床分离株5类,标准株1类)。内镜分析显示胃炎病例最有可能与D类相关,而与a类相关的病例没有。从日本分离株获得的PCR产物的模式与先前报道的vacA序列的理论模式进行了比较。测定各菌株vacA扩增片段的核苷酸序列,并与已有报道的vacA基因序列进行比较。
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Variations in the gene for vacuolating toxin, vacA, in Japanese isolates of Helicobacter pylori.

Each of 284 strains of Helicobacter pylori which had been isolated in Japan was shown, by use of the polymerase chain reaction (PCR), to be positive for the vacA genes. The amplified vacA genes generated by PCR were classified into six classes (five for the clinical isolates, and one which corresponded to the standard strains). Endoscopic analysis revealed that cases of gastritis were most likely to be associated with class D, while none were associated with class A. The patterns of products of PCR obtained from the Japanese isolates were compared with theoretical patterns derived from sequences of vacA which had been reported previously. The nucleotide sequences of amplified fragments of vacA from representative strains in each class were determined and compared with those of previously reported vacA genes.

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