埃塞俄比亚鸡中流行的传染性法氏囊病病毒的基因分组:将国内非常强毒的毒株分配到新亚基因组3d的建议

IF 1.7 Q2 VETERINARY SCIENCES Veterinary medicine (Auckland, N.Z.) Pub Date : 2021-02-26 eCollection Date: 2021-01-01 DOI:10.2147/VMRR.S296367
Fufa Dawo Bari
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引用次数: 4

摘要

2017年,传染性法氏囊病病毒(IBDVs)根据VP2基因序列数据构建的系统发育树的聚类性质重新分类,而不是根据其病原型和/或抗原类型。埃塞俄比亚IBD病毒没有根据提出的基因分组重新分类。方法:为了对埃塞俄比亚IBDVs进行基因分组,从GenBank中检索到可用的VP2基因序列数据和参考菌株序列,并根据进化树重建和系统发育树聚类的确定,按照最近推荐的方法进行基因分组。结果:埃塞俄比亚IBDVs被分为基因组1和基因组3,从抗原性上分别代表经典毒力和非常毒力IBDVs。基因组1的IBDVs与疫苗毒株聚类,而基因组3的病毒与亚基因组3a和亚基因组3b的4种已知病毒聚类。报告的埃塞俄比亚ibdv中几乎有一半不属于基因组3的特定亚群;相反,分离株聚类不同,表明它们应该有一个不同的亚基因组,暂定为3d。通过系统发育树聚类分析得出的两个基因群在VP2序列相似位置上的氨基酸变化得到了相应的支持。此外,在这些序列中,毒力标记氨基酸基因与第二主要亲水性区域(氨基酸位置314-325)偶联,预测可能是IBD爆发的原因。结论:在序列中观察到一个新的ibdv亚基因组3d,这可能是IBD暴发频繁发生的原因之一,并对现有疫苗的保护潜力提出质疑。为了在该国实施疾病控制,需要在体内评估正在使用的疫苗对在该国流行的第1和第3基因组病毒以及所有3个亚基因组病毒的有效性。
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Genogrouping of Infectious Bursal Disease Viruses Circulating in Ethiopian Chickens: Proposal for Assigning Very Virulent Strains in the Country into New Sub Genogroup 3d.

Introduction: In 2017 infectious bursal disease viruses (IBDVs) were reclassified into genogroups based on nature of clustering on a phylogenetic tree constructed using VP2 gene sequence data rather than according to their pathotype and/or antigenic types. Ethiopian IBD viruses were not reclassified according to the proposed genogrouping.

Methods: In order to genogroup the Ethiopian IBDVs, available VP2 gene sequences data together with reference strain sequences were retrieved from GenBank and genogrouped as recently recommended based on evolutionary tree reconstruction and determination of their clustering on the phylogenetic tree.

Results: The Ethiopian IBDVs were grouped into genogroups 1 and 3 that antigenically represent classically virulent and very virulent IBDVs, respectively. The genogroup 1 IBDVs were clustered with the vaccine strain while the genogroup 3 viruses were clustered with four known viruses belonging to sub-genogroup 3a and sub-genogroup 3b. Almost half of the Ethiopian IBDVs reported did not cluster with the specific sub-groups of genogroup 3; rather, the isolates were clustered differently suggesting they deserve a different sub-genogroup tentatively proposed as 3d. The two genogroups observed based on clustering on a phylogenetic tree were supported by corresponding deduced amino acid changes in similar positions in VP2 sequences. In addition, virulence marker amino acid genes coupled with second major hydrophilic region (amino acid positions 314-325) were predicted in these sequences that could be responsible for the occurrence of IBD outbreaks.

Conclusion: A new sub-genogroup of IBDVs, 3d, were observed in the sequences that could be one of the reasons for the frequent occurrence of IBD outbreaks and questions the protective potential of the existing vaccine. To institute disease control in the country, the effectiveness of the vaccine in use needs to be assessed in vivo against both genogroups 1 and 3 viruses and all three sub-genogroup 3 viruses circulating in the country.

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