改进接触x射线显微摄影法测量牙硬组织矿物质密度。

IF 1.5 4区 工程技术 Journal of Research of the National Institute of Standards and Technology Pub Date : 2010-04-01 Print Date: 2010-03-01 DOI:10.6028/jres.115.006
B D Schmuck, C M Carey
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引用次数: 9

摘要

接触式x射线显微摄影是目前测量部分脱矿牙齿标本矿物质密度的金标准。在jres NIST关于该主题的最新出版物中指定的x射线敏感胶片已不再在商业上可用。目的:通过识别对X射线敏感的大于3000线/毫米分辨率的市售胶片,开发一种新的微射线照相方法,并开发用于X射线微射线照相应用的正确胶片处理方法。方法:全息膜被确定为一种潜在的替代膜。适当的曝光是确定利用厚镍板创建测试条。胶卷的冲洗是围绕制造商的建议进行的。用铝阶梯楔测定薄膜线性度。100µm厚的牙齿切片的微射线照片,在酸性挑战之前和之后,是对胶片的最后测试。用分辨率为0.305微米/像素的数码显微镜相机拍摄放大图像。结果:适当的显影时间为2分钟,80 kV(p), 3 mA,曝光时间为30分钟。阶梯形实验表明,当归一化像素强度值为满量程的10%至90% (r(2) = 0.997)时,该系统的像素强度与x射线衰减呈线性关系,该归一化像素强度值包含牙齿组织的全暴露区域。牙釉质切片分析显示出侵蚀和脱矿的明显差异。每像素0.305微米的图像捕获设备分辨率限制了系统分辨率。结论:使用鉴定的全息胶片与所描述的处理修改相结合,导致了一种改进的用于测量牙齿硬组织矿物质密度的x射线显微放射学方法。通过使用更高分辨率的数字化系统,可以进一步改进所描述的方法。该方法适用于定量测量矿物密度和侵蚀的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Improved Contact X-Ray Microradiographic Method to Measure Mineral Density of Hard Dental Tissues.

Contact X-ray microradiography is the current gold standard for measuring mineral densities of partially demineralized tooth specimens. The X-ray sensitive film specified in the last J Res NIST publication on the subject is no longer commercially available. OBJECTIVES: Develop a new microradiographic method by identifying a commercially available film with greater than 3000 lines per millimeter resolution, which is sensitive to X rays, and develop correct film processing for X-ray microradiographic application. METHODS: A holographic film was identified as a potential replacement film. Proper exposure was determined utilizing a thick nickel plate to create test-strips. Film development was bracketed around manufacturer suggestions. Film linearity was determined with aluminum step-wedges. Microradiographs of 100 µm thick tooth sections, before and after acidic challenges, were a final test for film. Magnified images were captured with a digital microscope camera with 0.305 micrometers per pixel resolution. RESULTS: The appropriate film exposure was 30 minutes at 80 kV(p) and 3 mA with a development time of 2 minutes. Step-wedge experiments show the system to be linear in terms of pixel intensities with respect to x-ray attenuation for normalized pixel intensity values that are 10% to 90% of full scale (r(2) = 0.997) which encompasses the full exposure region of tooth tissue. Enamel sections were analyzed and show distinctive differences between erosion and demineralization. The image capture device resolution of 0.305 micrometers per pixel limits the system resolution. CONCLUSION: Use of the identified holographic film when combined with the described processing modifications has resulted in an improved X-ray microradiographic method for the measurement of mineral density of dental hard tissues. The method described can be further improved by using a higher resolution digitization system. The method is appropriate for quantitatively measuring changes in mineral density and erosion.

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自引率
33.30%
发文量
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期刊介绍: The Journal of Research of the National Institute of Standards and Technology is the flagship publication of the National Institute of Standards and Technology. It has been published under various titles and forms since 1904, with its roots as Scientific Papers issued as the Bulletin of the Bureau of Standards. In 1928, the Scientific Papers were combined with Technologic Papers, which reported results of investigations of material and methods of testing. This new publication was titled the Bureau of Standards Journal of Research. The Journal of Research of NIST reports NIST research and development in metrology and related fields of physical science, engineering, applied mathematics, statistics, biotechnology, information technology.
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