培养H4IIE大鼠细胞中外源代谢酶对铅暴露的生物学反应。

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES Japanese Journal of Veterinary Research Pub Date : 2013-02-01

Wageh Sobhy Darwish, Yoshinori Ikenaka, Mayumi Ishizuka

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引用次数: 0

摘要

本研究旨在探讨培养大鼠肝(H4IIE)细胞系中外源代谢酶(XMEs)对铅(Pb2+)暴露的组成性反应。暴露于低浓度Pb2+后，CYP1A1和CYP1A2等I期酶的mRNA表达被轻微诱导;而高浓度Pb2+作用下，CYP1A1和CYP1A2 mRNA表达量显著下调。这些影响与AhR mRNA的表达一致。II期酶的mRNA表达在暴露于Pb2+后降低。Pb2+诱导金属硫蛋白mRNA表达呈剂量依赖性。综上所述，I期和II期酶在铅暴露后显著调节，这表明铅暴露对培养的H4IIE细胞具有一定的毒理学意义。

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Biological responses of xenobiotic metabolizing enzymes to lead exposure in cultured H4IIE rat cells.

This study was undertaken to investigate the constitutive response of xenobiotic metabolizing enzymes (XMEs) to lead (Pb2+) exposure in cultured rat liver (H4IIE) cell lines. Phase I enzymes such as CYP1A1 and CYP1A2 had mRNA expressions that were slightly induced after exposure to low concentrations of Pb2+; however, under higher concentrations of Pb2+, the mRNA expressions of CYP1A1 and CYP1A2 were significantly down-regulated. These effects were in correspondence with AhR mRNA expression. Phase II enzymes had mRNA expressions that were reduced upon exposure to Pb2+. Metallothionein mRNA expression was induced after treatment with Pb2+ in a dose-dependent trend. In conclusion, Phase I and II enzymes were significantly modulated upon lead exposure indicating some toxicological implications for lead exposure in cultured H4IIE cells.

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来源期刊

Japanese Journal of Veterinary Research 农林科学-兽医学

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Japanese Journal of Veterinary Research (JJVR) quarterly publishes peer-reviewed articles on all aspects of veterinary science. JJVR was originally published as a “University Journal” of veterinary science at Hokkaido University from more than 60 years ago. Currently, JJVR, is Japan’s leading scientific veterinary journal, and provides valuable information for the development of veterinary science by welcoming contributions from researchers worldwide. JJVR offers online submission for Regular Papers, Short Communications, and Review Articles that are unpublished and not being considered for publication elsewhere. Research areas include: Anatomy, Physiology, Biochemistry, Pharmacology, Microbiology, Infectious diseases, Parasitology, Laboratory Animal Science and Medicine, Internal Medicine, Surgery, Pathology, Theriogenology, Molecular Medicine, Public Health, Radiation Biology, Toxicology, Wildlife Biology and Medicine, Veterinary Hygiene, The other fields related to veterinary science.