肿瘤坏死因子和淋巴毒素的测定

Q2 Immunology and Microbiology Current Protocols in Immunology Pub Date : 2017-04-03 DOI:10.1002/cpim.23

M. Michele Hogan, Stefanie N. Vogel

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摘要

肿瘤坏死因子(TNF)细胞因子超家族在免疫应答的各个方面起着至关重要的作用。TNF和淋巴毒素(LT)， LTα和LTβ，作为淋巴器官发育的主要效应细胞因子和介质尤为重要。测量TNF和LTα活性的经典方法之一是通过证明它们裂解某些靶细胞的能力。提出了一种使用高度敏感的指示细胞系测量这种活性的详细方案。最近，酶联免疫吸附试验已经发展到测量这些细胞因子的蛋白质浓度在任何类型的生物液体。©2017 by John Wiley &儿子,Inc。

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Measurement of Tumor Necrosis Factor and Lymphotoxins

The tumor necrosis factor (TNF) superfamily of cytokines plays critical roles in all aspects of the immune response. TNF and the lymphotoxins (LT), LTα and LTβ, are particularly important as major effector cytokines and mediators or lymphoid organ development. One of the classical methods for the measurement of TNF and LTα activity is by demonstrating their ability to lyse certain target cells. A detailed protocol for the measurement of this activity using a highly sensitive indicator cell line is presented. More recently, ELISA assays have been developed to measure the protein concentration of these cytokines in any type of biologic fluid. © 2017 by John Wiley & Sons, Inc.

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Current Protocols in Immunology Immunology and Microbiology-Immunology

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