日本传统药物goshajinkagan抑制C2C12细胞中电压门控钠通道Nav1.4电流。

Q2 Biochemistry, Genetics and Molecular Biology BioResearch Open Access Pub Date : 2020-04-27 eCollection Date: 2020-01-01 DOI:10.1089/biores.2019.0034

Ryota Imai, Shoichiro Horita, Yuko Ono, Keisuke Hagihara, Masaru Shimizu, Yuko Maejima, Kenju Shimomura

{"title":"日本传统药物goshajinkagan抑制C2C12细胞中电压门控钠通道Nav1.4电流。","authors":"Ryota Imai, Shoichiro Horita, Yuko Ono, Keisuke Hagihara, Masaru Shimizu, Yuko Maejima, Kenju Shimomura","doi":"10.1089/biores.2019.0034","DOIUrl":null,"url":null,"abstract":"Goshajinkigan (GJG) is a traditional Japanese Kampo medicine used clinically to treat muscle pain in Japan. However, its underlying mechanism remains unclear. Since voltage-gated sodium channel (Nav) 1.4 is involved in skeletal muscle contraction, we investigated the possibility that GJG may affect Nav1.4 currents. By using an electrophysiological technique on skeletal muscle cell line C2C12, we found that GJG suppresses Nav1.4 currents in C2C12 cells. It is suggested that GJG may improve skeletal muscle stiffness or cramps by inhibiting abnormal Nav1.4 excitation. GJG may act as a Nav1.4 blocker and may be useful to treat muscle stiffness and clamps as well as easing the pain.","PeriodicalId":9100,"journal":{"name":"BioResearch Open Access","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/biores.2019.0034","citationCount":"2","resultStr":"{\"title\":\"Goshajinkigan, a Traditional Japanese Medicine, Suppresses Voltage-Gated Sodium Channel Nav1.4 Currents in C2C12 Cells.\",\"authors\":\"Ryota Imai, Shoichiro Horita, Yuko Ono, Keisuke Hagihara, Masaru Shimizu, Yuko Maejima, Kenju Shimomura\",\"doi\":\"10.1089/biores.2019.0034\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Goshajinkigan (GJG) is a traditional Japanese Kampo medicine used clinically to treat muscle pain in Japan. However, its underlying mechanism remains unclear. Since voltage-gated sodium channel (Nav) 1.4 is involved in skeletal muscle contraction, we investigated the possibility that GJG may affect Nav1.4 currents. By using an electrophysiological technique on skeletal muscle cell line C2C12, we found that GJG suppresses Nav1.4 currents in C2C12 cells. It is suggested that GJG may improve skeletal muscle stiffness or cramps by inhibiting abnormal Nav1.4 excitation. GJG may act as a Nav1.4 blocker and may be useful to treat muscle stiffness and clamps as well as easing the pain.\",\"PeriodicalId\":9100,\"journal\":{\"name\":\"BioResearch Open Access\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-04-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1089/biores.2019.0034\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BioResearch Open Access\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/biores.2019.0034\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BioResearch Open Access","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/biores.2019.0034","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 2

摘要

Goshajinkigan (GJG)是一种传统的日本汉布药，在日本临床上用于治疗肌肉疼痛。然而，其潜在机制尚不清楚。由于电压门控钠通道(Nav) 1.4参与骨骼肌收缩，我们研究了GJG可能影响Nav1.4电流的可能性。利用电生理技术对C2C12骨骼肌细胞系进行实验，发现GJG对C2C12细胞的Nav1.4电流有抑制作用。提示GJG可能通过抑制Nav1.4异常兴奋来改善骨骼肌僵硬或痉挛。GJG可能作为Nav1.4阻滞剂，可能对治疗肌肉僵硬和夹痛以及缓解疼痛有用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

摘要图片

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Goshajinkigan, a Traditional Japanese Medicine, Suppresses Voltage-Gated Sodium Channel Nav1.4 Currents in C2C12 Cells.

Goshajinkigan (GJG) is a traditional Japanese Kampo medicine used clinically to treat muscle pain in Japan. However, its underlying mechanism remains unclear. Since voltage-gated sodium channel (Nav) 1.4 is involved in skeletal muscle contraction, we investigated the possibility that GJG may affect Nav1.4 currents. By using an electrophysiological technique on skeletal muscle cell line C2C12, we found that GJG suppresses Nav1.4 currents in C2C12 cells. It is suggested that GJG may improve skeletal muscle stiffness or cramps by inhibiting abnormal Nav1.4 excitation. GJG may act as a Nav1.4 blocker and may be useful to treat muscle stiffness and clamps as well as easing the pain.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

BioResearch Open Access Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

自引率

0.00%

发文量

期刊介绍： BioResearch Open Access is a high-quality open access journal providing peer-reviewed research on a broad range of scientific topics, including molecular and cellular biology, tissue engineering, regenerative medicine, stem cells, gene therapy, systems biology, genetics, virology, and neuroscience. The Journal publishes basic science and translational research in the form of original research articles, comprehensive review articles, mini-reviews, rapid communications, brief reports, technology reports, hypothesis articles, perspectives, and letters to the editor.