吲哚菁绿实时荧光成像评价近红外光免疫治疗肿瘤模型小鼠的疗效。

IF 2.8 4区 医学 Q2 Medicine Molecular Imaging Pub Date : 2020-01-01 DOI:10.1177/1536012120934965
Adrian Rosenberg, Daiki Fujimura, Ryuhei Okada, Aki Furusawa, Fuyuki Inagaki, Hiroaki Wakiyama, Takuya Kato, Peter L Choyke, Hisataka Kobayashi
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引用次数: 4

摘要

背景:近红外光免疫疗法(NIR-PIT)是一种引起肿瘤灌注增加的癌症治疗方法,这种现象被称为超增强的通透性和滞留效应。目前,NIR-PIT的体内治疗效果是在治疗后几天观察到的,但通过更急性地检测肿瘤内的变化,监测将得到改善。荧光成像可在治疗后立即发现肿瘤灌注增加。方法:在第一个实验中,胸腺裸鼠单侧皮下肿瘤模型分别用NIR-PIT或激光治疗。在第二个实验中,携带双侧侧腹肿瘤的小鼠仅在左侧肿瘤上进行NIR-PIT治疗。两组治疗后立即静脉注射不同剂量的吲哚菁绿,用岛津LIGHTVISION荧光成像系统监测小鼠1小时。结果:在60分钟的监测中,治疗小鼠的肿瘤与背景的荧光强度之比增加,但在对照组小鼠中没有显著变化。在1 mg kg-1和0.3 mg kg-1剂量下,肿瘤与背景比最高。在双侧肿瘤小鼠中,肿瘤与未治疗的肿瘤比例也同样增加。结论:实时荧光成像可检测NIR-PIT术后肿瘤灌注的急性变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice.

Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment.

Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour.

Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg-1 and 0.3 mg kg-1 doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly.

Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.

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来源期刊
Molecular Imaging
Molecular Imaging 生物-核医学
CiteScore
4.50
自引率
3.60%
发文量
21
审稿时长
>12 weeks
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
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