吲哚菁绿实时荧光成像评价近红外光免疫治疗肿瘤模型小鼠的疗效。

IF 2.8 4区 医学 Q2 Medicine Molecular Imaging Pub Date : 2020-01-01 DOI:10.1177/1536012120934965
Adrian Rosenberg, Daiki Fujimura, Ryuhei Okada, Aki Furusawa, Fuyuki Inagaki, Hiroaki Wakiyama, Takuya Kato, Peter L Choyke, Hisataka Kobayashi
{"title":"吲哚菁绿实时荧光成像评价近红外光免疫治疗肿瘤模型小鼠的疗效。","authors":"Adrian Rosenberg,&nbsp;Daiki Fujimura,&nbsp;Ryuhei Okada,&nbsp;Aki Furusawa,&nbsp;Fuyuki Inagaki,&nbsp;Hiroaki Wakiyama,&nbsp;Takuya Kato,&nbsp;Peter L Choyke,&nbsp;Hisataka Kobayashi","doi":"10.1177/1536012120934965","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment.</p><p><strong>Methods: </strong>In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour.</p><p><strong>Results: </strong>Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg<sup>-1</sup> and 0.3 mg kg<sup>-1</sup> doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly.</p><p><strong>Conclusions: </strong>Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.</p>","PeriodicalId":49796,"journal":{"name":"Molecular Imaging","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1536012120934965","citationCount":"4","resultStr":"{\"title\":\"Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice.\",\"authors\":\"Adrian Rosenberg,&nbsp;Daiki Fujimura,&nbsp;Ryuhei Okada,&nbsp;Aki Furusawa,&nbsp;Fuyuki Inagaki,&nbsp;Hiroaki Wakiyama,&nbsp;Takuya Kato,&nbsp;Peter L Choyke,&nbsp;Hisataka Kobayashi\",\"doi\":\"10.1177/1536012120934965\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment.</p><p><strong>Methods: </strong>In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour.</p><p><strong>Results: </strong>Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg<sup>-1</sup> and 0.3 mg kg<sup>-1</sup> doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly.</p><p><strong>Conclusions: </strong>Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.</p>\",\"PeriodicalId\":49796,\"journal\":{\"name\":\"Molecular Imaging\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2020-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1177/1536012120934965\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Imaging\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/1536012120934965\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Imaging","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/1536012120934965","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 4

摘要

背景:近红外光免疫疗法(NIR-PIT)是一种引起肿瘤灌注增加的癌症治疗方法,这种现象被称为超增强的通透性和滞留效应。目前,NIR-PIT的体内治疗效果是在治疗后几天观察到的,但通过更急性地检测肿瘤内的变化,监测将得到改善。荧光成像可在治疗后立即发现肿瘤灌注增加。方法:在第一个实验中,胸腺裸鼠单侧皮下肿瘤模型分别用NIR-PIT或激光治疗。在第二个实验中,携带双侧侧腹肿瘤的小鼠仅在左侧肿瘤上进行NIR-PIT治疗。两组治疗后立即静脉注射不同剂量的吲哚菁绿,用岛津LIGHTVISION荧光成像系统监测小鼠1小时。结果:在60分钟的监测中,治疗小鼠的肿瘤与背景的荧光强度之比增加,但在对照组小鼠中没有显著变化。在1 mg kg-1和0.3 mg kg-1剂量下,肿瘤与背景比最高。在双侧肿瘤小鼠中,肿瘤与未治疗的肿瘤比例也同样增加。结论:实时荧光成像可检测NIR-PIT术后肿瘤灌注的急性变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice.

Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment.

Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour.

Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg-1 and 0.3 mg kg-1 doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly.

Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular Imaging
Molecular Imaging 生物-核医学
CiteScore
4.50
自引率
3.60%
发文量
21
审稿时长
>12 weeks
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
期刊最新文献
Magnetic Particle Imaging in Vascular Imaging, Immunotherapy, Cell Tracking, and Noninvasive Diagnosis Development and Evaluation of a Novel Radiotracer 125I-rIL-27 to Monitor Allotransplant Rejection by Specifically Targeting IL-27Rα 13-cis-Retinoic Acid Affects Brain Perfusion and Function: In Vivo Study Immuno-PET Imaging of Siglec-15 Using the Zirconium-89-Labeled Therapeutic Antibody, NC318 Pattern of F-18 FDG Uptake in Colon Cancer after Bacterial Cancer Therapy Using Engineered Salmonella Typhimurium: A Preliminary In Vivo Study
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1