Janne Cadamuro, Peter Bergsten, Katharina Mörwald, Anders Forslund, Marie Dahlbom, Jonas Bergquist, Iris Ciba, Susanne M Brunner, Jeanne Jabbour, Daniel Weghuber
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引用次数: 0
摘要
在一项针对肥胖和正常体重儿童和青少年的双中心研究中,我们观察到两个地点(奥地利萨尔茨堡和瑞典乌普萨拉)的葡萄糖结果有显著差异(N = 16,840) (P < 0.001)。在排除了两个人群之间患者特征的差异作为这一发现的原因后,我们调查了其他分析前影响。最后,只留下两个地点用于采血的管子进行评估。乌普萨拉使用Vacuette FC-Mix管(Greiner Bio-One, kremsmnster,奥地利),在萨尔茨堡采集血液时使用锂肝素管(hl - monovette,德国萨斯泰特)。为了证明我们的假设,我们从51名儿童(Salzburg N = 27, Uppsala N = 24)中采集了两份血样,并比较了测量的葡萄糖结果。事实上,我们发现了可疑的偏倚,并计算了一个校正公式,显著减小了两个地点之间葡萄糖结果的差异(P = 0.023)。我们的发现与其他研究一致,尽管这个问题应该广为人知,但我们觉得它被广泛忽视了,特别是在比较欧洲各地的葡萄糖浓度时,使用大型数据库而没有任何分析前样品处理的信息。
Deviating glucose results in an international dual-center study. A root cause investigation.
During a dual-center study on obese and normal weight children and adolescents, focusing on glucose metabolism, we observed a marked difference in glucose results (N = 16,840) between the two sites, Salzburg, Austria and Uppsala, Sweden (P < 0.001). After excluding differences in patient characteristics between the two populations as cause of this finding, we investigated other preanalytic influences. Finally, only the tubes used for blood collection at the two sites were left to evaluate. While the Vacuette FC-Mix tube (Greiner Bio-One, Kremsmünster, Austria) was used in Uppsala, in Salzburg blood collections were performed with a lithium heparin tube (LH-Monovette, Sarstedt, Germany). To prove our hypothesis, we collected two blood samples in either of these tubes from 51 children (Salzburg N = 27, Uppsala N = 24) and compared the measured glucose results. Indeed, we found the suspected bias and calculated a correction formula, which significantly diminished the differences of glucose results between the two sites (P = 0.023). Our finding is in line with those of other studies and although this issue should be widely known, we feel that it is widely neglected, especially when comparing glucose concentrations across Europe, using large databases without any information on preanalytic sample handling.
期刊介绍:
Biochemia Medica is the official peer-reviewed journal of the Croatian Society of Medical Biochemistry and Laboratory Medicine. Journal provides a wide coverage of research in all aspects of clinical chemistry and laboratory medicine. Following categories fit into the scope of the Journal: general clinical chemistry, haematology and haemostasis, molecular diagnostics and endocrinology. Development, validation and verification of analytical techniques and methods applicable to clinical chemistry and laboratory medicine are welcome as well as studies dealing with laboratory organization, automation and quality control. Journal publishes on a regular basis educative preanalytical case reports (Preanalytical mysteries), articles dealing with applied biostatistics (Lessons in biostatistics) and research integrity (Research integrity corner).