{"title":"三叶草提取物提高糖尿病大鼠睾酮水平，改善精子特征和抗氧化状态。","authors":"Mohammad Rasool Khazaei, Elham Gravandi, Elham Ghanbari, Elham Niromand, Mozafar Khazaei","doi":"10.1080/10520295.2022.2039766","DOIUrl":null,"url":null,"abstract":"<p><p>Male reproductive dysfunction is a common complication of diabetes mellitus. <i>Trifolium pratense</i> exhibits antioxidant and antidiabetic effects. We investigated the effects of an extract of <i>T. pratense</i> on serum antioxidant status, sperm characteristics, testicular tissue changes and testosterone level in diabetic rats. Male Wistar rats were divided into six groups: 1, untreated control; 2, diabetic; 3 and 4, 100 or 200 mg/kg <i>T. pratense</i> extract treated, respectively; 5 and 6, diabetic 100 or 200 mg/kg <i>T. pratense</i> extract treated, respectively. Diabetes was induced by intraperitoneal injection of streptozotocin. After 3 weeks, serum glucose, testosterone and nitric oxide (NO); sperm parameters; testicular histology and total antioxidant capacity (TAC) were evaluated. In diabetic rats treated with <i>T. pratense</i> extract, sperm motility, count and viability, as well as TAC and testosterone were increased significantly compared to untreated diabetic rats, while serum NO and bcl-2 and p53 expression was decreased significantly compared to untreated diabetic rats. <i>T. pratense</i> extract reduced testicular tissue destruction caused by diabetes.</p>","PeriodicalId":8970,"journal":{"name":"Biotechnic & Histochemistry","volume":"97 8","pages":"576-583"},"PeriodicalIF":1.6000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"<i>Trifolium pratense</i> extract increases testosterone and improves sperm characteristics and antioxidant status in diabetic rats.\",\"authors\":\"Mohammad Rasool Khazaei, Elham Gravandi, Elham Ghanbari, Elham Niromand, Mozafar Khazaei\",\"doi\":\"10.1080/10520295.2022.2039766\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Male reproductive dysfunction is a common complication of diabetes mellitus. <i>Trifolium pratense</i> exhibits antioxidant and antidiabetic effects. We investigated the effects of an extract of <i>T. pratense</i> on serum antioxidant status, sperm characteristics, testicular tissue changes and testosterone level in diabetic rats. Male Wistar rats were divided into six groups: 1, untreated control; 2, diabetic; 3 and 4, 100 or 200 mg/kg <i>T. pratense</i> extract treated, respectively; 5 and 6, diabetic 100 or 200 mg/kg <i>T. pratense</i> extract treated, respectively. Diabetes was induced by intraperitoneal injection of streptozotocin. After 3 weeks, serum glucose, testosterone and nitric oxide (NO); sperm parameters; testicular histology and total antioxidant capacity (TAC) were evaluated. In diabetic rats treated with <i>T. pratense</i> extract, sperm motility, count and viability, as well as TAC and testosterone were increased significantly compared to untreated diabetic rats, while serum NO and bcl-2 and p53 expression was decreased significantly compared to untreated diabetic rats. <i>T. pratense</i> extract reduced testicular tissue destruction caused by diabetes.</p>\",\"PeriodicalId\":8970,\"journal\":{\"name\":\"Biotechnic & Histochemistry\",\"volume\":\"97 8\",\"pages\":\"576-583\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnic & Histochemistry\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1080/10520295.2022.2039766\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/2/14 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnic & Histochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/10520295.2022.2039766","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/2/14 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Trifolium pratense extract increases testosterone and improves sperm characteristics and antioxidant status in diabetic rats.
Male reproductive dysfunction is a common complication of diabetes mellitus. Trifolium pratense exhibits antioxidant and antidiabetic effects. We investigated the effects of an extract of T. pratense on serum antioxidant status, sperm characteristics, testicular tissue changes and testosterone level in diabetic rats. Male Wistar rats were divided into six groups: 1, untreated control; 2, diabetic; 3 and 4, 100 or 200 mg/kg T. pratense extract treated, respectively; 5 and 6, diabetic 100 or 200 mg/kg T. pratense extract treated, respectively. Diabetes was induced by intraperitoneal injection of streptozotocin. After 3 weeks, serum glucose, testosterone and nitric oxide (NO); sperm parameters; testicular histology and total antioxidant capacity (TAC) were evaluated. In diabetic rats treated with T. pratense extract, sperm motility, count and viability, as well as TAC and testosterone were increased significantly compared to untreated diabetic rats, while serum NO and bcl-2 and p53 expression was decreased significantly compared to untreated diabetic rats. T. pratense extract reduced testicular tissue destruction caused by diabetes.
期刊介绍：
Biotechnic & Histochemistry (formerly Stain technology) is the
official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926.
Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well.
Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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