人b淋巴母细胞样TK6细胞II期药物代谢酶的表达。

IF 1.2 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2022-01-01 Epub Date: 2022-03-11 DOI:10.1080/26896583.2022.2044242
Xilin Li, Yuxi Li, Kylie G Ning, Si Chen, Lei Guo, Jessica A Bonzo, Nan Mei
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引用次数: 1

摘要

体外遗传毒性检测在化学品风险评估中起着重要作用。人b淋巴母细胞样细胞系TK6被广泛用作监管安全性评估的标准细胞系。像许多其他哺乳动物细胞系一样,TK6细胞的代谢能力有限;因此,通常需要外源性代谢激活源用于遗传毒性试验。此前,我们开发了一组tk6衍生的细胞系,这些细胞系分别表达14种细胞色素p450 (CYPs)中的一种。在本研究中,我们调查了一组主要的II期药物代谢酶,以表征它们在TK6细胞中的基线表达。这些结果可以作为这种常用细胞系的参考酶谱。
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The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells.

In vitro genotoxicity testing plays an important role in chemical risk assessment. The human B-lymphoblastoid cell line TK6 is widely used as a standard cell line for regulatory safety evaluations. Like many other mammalian cell lines, TK6 cells have limited metabolic capacity; therefore, usually require a source of exogenous metabolic activation for use in genotoxicity testing. Previously, we developed a set of TK6-derived cell lines that individually express one of fourteen cytochrome P450s (CYPs). In the present study, we surveyed a panel of major Phase II drug-metabolizing enzymes to characterize their baseline expression in TK6 cells. These results may serve as a reference enzymatic profile of this commonly used cell line.

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