H Ghobadian Diali, H Hosseini, M H Fallah Mehrabadi, R Yahyaraeyat, A Ghalyanchilangeroudi
{"title":"传染性支气管炎病毒攻毒后两种商品肉鸡杂交鸡组织中病毒载量和凋亡基因表达水平的比较研究","authors":"H Ghobadian Diali, H Hosseini, M H Fallah Mehrabadi, R Yahyaraeyat, A Ghalyanchilangeroudi","doi":"10.22099/IJVR.2021.35894.5261","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Infectious bronchitis virus (IBV) causes severe economic losses worldwide. IBV has a broad tissue distribution with different viral loads in different tissues. Additionally, IBV can induce apoptosis in infected cells.</p><p><strong>Aims: </strong>The present study aimed to evaluate the role of the genetic background of chickens in viral load and the expression level of apoptotic genes in different tissues of two hybrids of commercial broiler chickens (Ross 308 and Cobb 500) challenged with IBV.</p><p><strong>Methods: </strong>Chickens at 21 days of age were nasally challenged with 200 μL of allantoic fluid containing 10<sup>4</sup> EID<sub>50</sub>/ml of Iranian variant-2-like IBV (IS/1494). The expression level of apoptotic genes (<i>Fas</i>, <i>FasL</i>, <i>Bax</i>, and <i>Bcl-2</i>) in the tracheal and renal tissues and the amount of viral load in the tracheal, renal, and cloacal swab samples were investigated two, five, and seven days after IBV infection by RT-qPCR assay.</p><p><strong>Results: </strong>The amount of viral load and apoptotic the expression level of apoptotic genes in the tracheal (two and five days after infection) and renal samples (seven days after infection) were significantly higher in the Ross challenged group than in the Cobb challenged group.Furthermore, no difference was observed in the cloaca viral load on sampling days.</p><p><strong>Conclusion: </strong>To our knowledge, this is the first report that evaluated the role of the chickens' genetic background in the amount of viral load and the expression level of apoptotic genes against IBV. Further studies are needed to investigate the pathogenic characteristics of IBV in Ross 308 and Cobb 500 chickens.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"23 1","pages":"18-23"},"PeriodicalIF":0.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238929/pdf/ijvr-23-18.pdf","citationCount":"0","resultStr":"{\"title\":\"Evaluation of viral load and expression level of apoptotic genes in selected tissues of two hybrids of commercial broiler chickens challenged with infectious bronchitis virus: a comparative study.\",\"authors\":\"H Ghobadian Diali, H Hosseini, M H Fallah Mehrabadi, R Yahyaraeyat, A Ghalyanchilangeroudi\",\"doi\":\"10.22099/IJVR.2021.35894.5261\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Infectious bronchitis virus (IBV) causes severe economic losses worldwide. IBV has a broad tissue distribution with different viral loads in different tissues. Additionally, IBV can induce apoptosis in infected cells.</p><p><strong>Aims: </strong>The present study aimed to evaluate the role of the genetic background of chickens in viral load and the expression level of apoptotic genes in different tissues of two hybrids of commercial broiler chickens (Ross 308 and Cobb 500) challenged with IBV.</p><p><strong>Methods: </strong>Chickens at 21 days of age were nasally challenged with 200 μL of allantoic fluid containing 10<sup>4</sup> EID<sub>50</sub>/ml of Iranian variant-2-like IBV (IS/1494). The expression level of apoptotic genes (<i>Fas</i>, <i>FasL</i>, <i>Bax</i>, and <i>Bcl-2</i>) in the tracheal and renal tissues and the amount of viral load in the tracheal, renal, and cloacal swab samples were investigated two, five, and seven days after IBV infection by RT-qPCR assay.</p><p><strong>Results: </strong>The amount of viral load and apoptotic the expression level of apoptotic genes in the tracheal (two and five days after infection) and renal samples (seven days after infection) were significantly higher in the Ross challenged group than in the Cobb challenged group.Furthermore, no difference was observed in the cloaca viral load on sampling days.</p><p><strong>Conclusion: </strong>To our knowledge, this is the first report that evaluated the role of the chickens' genetic background in the amount of viral load and the expression level of apoptotic genes against IBV. Further studies are needed to investigate the pathogenic characteristics of IBV in Ross 308 and Cobb 500 chickens.</p>\",\"PeriodicalId\":14629,\"journal\":{\"name\":\"Iranian journal of veterinary research\",\"volume\":\"23 1\",\"pages\":\"18-23\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238929/pdf/ijvr-23-18.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian journal of veterinary research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.22099/IJVR.2021.35894.5261\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2021.35894.5261","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Evaluation of viral load and expression level of apoptotic genes in selected tissues of two hybrids of commercial broiler chickens challenged with infectious bronchitis virus: a comparative study.
Background: Infectious bronchitis virus (IBV) causes severe economic losses worldwide. IBV has a broad tissue distribution with different viral loads in different tissues. Additionally, IBV can induce apoptosis in infected cells.
Aims: The present study aimed to evaluate the role of the genetic background of chickens in viral load and the expression level of apoptotic genes in different tissues of two hybrids of commercial broiler chickens (Ross 308 and Cobb 500) challenged with IBV.
Methods: Chickens at 21 days of age were nasally challenged with 200 μL of allantoic fluid containing 104 EID50/ml of Iranian variant-2-like IBV (IS/1494). The expression level of apoptotic genes (Fas, FasL, Bax, and Bcl-2) in the tracheal and renal tissues and the amount of viral load in the tracheal, renal, and cloacal swab samples were investigated two, five, and seven days after IBV infection by RT-qPCR assay.
Results: The amount of viral load and apoptotic the expression level of apoptotic genes in the tracheal (two and five days after infection) and renal samples (seven days after infection) were significantly higher in the Ross challenged group than in the Cobb challenged group.Furthermore, no difference was observed in the cloaca viral load on sampling days.
Conclusion: To our knowledge, this is the first report that evaluated the role of the chickens' genetic background in the amount of viral load and the expression level of apoptotic genes against IBV. Further studies are needed to investigate the pathogenic characteristics of IBV in Ross 308 and Cobb 500 chickens.
期刊介绍：
The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.
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