不孕与受精β蛋白分布有关系吗?

IF 0.8 4区医学 Q4 ANDROLOGY Revista Internacional De Andrologia Pub Date : 2022-10-01 DOI:10.1016/j.androl.2021.04.003

Gul Ipek Gundogan , Tulay Irez , Hasan Hakan Bozkurt

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引用次数: 1

摘要

受精素β是一种精子表面蛋白，可介导精子-卵子膜相互作用。本研究旨在探讨经标准精液分析后参数正常的供体在宫内人工授精(IUI)后受精蛋白β的表达是否与受精成功率低或失败有关。方法对30例以人工授精为临床指征的正常、少精症和不明原因不育症男性捐精者进行精子检测。用间接免疫荧光法(IF)标记了ADAM2抗体。为了评价试验的再现性，我们根据荧光标记的分布选取了4个精子样本，分值为0 ~ +++。结果结果与校正总细胞荧光(CTCF)高度相关(Rp = 0.9972, P <0.05)。我们认为不孕和受精β之间的关系可能是由于这种蛋白质在精子表面的分布。不明原因不孕症男性伴侣的IF标记荧光信号(+++标度)下降7.4±10.32%，P <0.0001±SD)。受精β功能异常可能是导致受精失败的潜在机制。

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Is there a relationship between infertility and fertilin β protein distribution?

Introduction

Fertilin β is a sperm surface protein that can mediate sperm-egg membrane interaction. This study was conducted to determine whether the expression of fertilin β after intrauterine insemination (IUI) in donors with normal parameters after standard semen analysis is related to low success rate or failure of fertilization.

Methods

We examined the sperm of 30 male donors who have normal as controls, oligozoospermia, and unexplained infertility as the clinically indication for IUI. Fertilin β has been labeled with the ADAM2 antibody by indirect immunofluorescence (IF) assay. To evaluate the reproducibility of the test, we selected four sperm samples scale of 0 to +++ according to the distribution of fluorescence label.

Results

The results were highly correlated with the corrected total cell fluorescence (CTCF) (R_p = 0.9972, P < 0.05). We suggest that the relationship between infertility and fertilin β may be due to the distribution of this protein on the sperm surface. Male partners of couples with unexplained infertility showed a low distribution of fertilin β by a decrease of the fluorescence signal in the IF labeling (scale of +++ by 7.4 ± 10.32%, P < 0.0001, ±SD).

Discussion

Abnormal fertilin β function may be a potential mechanism that could lead to fertilization failure.

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来源期刊

Revista Internacional De Andrologia ANDROLOGY-

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

>12 weeks

期刊介绍： Revista Internacional de Andrología es la revista oficial de la Asociación Española de Andrología, Medicina Sexual y Reproductiva (ASESA), la Sociedade Portuguesa de Ardrologia, la Sociedad Argentina de Andrología (SAA), la Asociación Iberoamericana de Sociedades de Andrología (ANDRO), y la Federación Española de Sociedades de Sexología. La revista publicada trimestralmente es revisada por pares y es líder en el la especialidad y en español y portugués. Recientemente también publica artículos en inglés. El objetivo de la revista es principalmente la promoción del conocimiento y la educación médica continua, con un enfoque especial en el público español y latinoamericano, a través de la publicación de las contribuciones importantes de la investigación en el campo. Todos los miembros de las sociedades antes mencionadas reciben la revista y otros suscriptores individuales e institucionales de España, Portugal y América Latina.