t细胞免疫球蛋白和粘蛋白结构域3在类风湿关节炎中上调,但在控制炎症方面不足。

IF 1.4 Q4 IMMUNOLOGY American journal of clinical and experimental immunology Pub Date : 2022-06-15 eCollection Date: 2022-01-01
Caecilie Skejoe, Aida S Hansen, Kristian Stengaard-Pedersen, Peter Junker, Kim Hoerslev-Pedersen, Merete L Hetland, Mikkel Oestergaard, Stinne Greisen, Malene Hvid, Mette Deleuran, Bent Deleuran
{"title":"t细胞免疫球蛋白和粘蛋白结构域3在类风湿关节炎中上调,但在控制炎症方面不足。","authors":"Caecilie Skejoe,&nbsp;Aida S Hansen,&nbsp;Kristian Stengaard-Pedersen,&nbsp;Peter Junker,&nbsp;Kim Hoerslev-Pedersen,&nbsp;Merete L Hetland,&nbsp;Mikkel Oestergaard,&nbsp;Stinne Greisen,&nbsp;Malene Hvid,&nbsp;Mette Deleuran,&nbsp;Bent Deleuran","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Rheumatoid arthritis (RA) is a chronic autoimmune disease, that involves both pro- and anti-inflammatory mechanisms. The purpose of the present study is to investigate T-cell immunoglobulin and mucin domain 3 (Tim-3) in RA.</p><p><strong>Methods: </strong>Plasma levels of soluble (s) Tim-3 in early RA (n=98), were followed, to evaluate association with treatment and disease activity, acquired from a prospective collected biobank (clinicaltrials.gov (NCT00660647)). We also investigate the influence of Tim-3 on spontaneous cytokine production in synovial fluid mononuclear cells (SFMC) from RA patients after addition of neutralizing anti-Tim-3's antibodies, either alone or in combination with neutralizing anti-Programmed Cell death protein 1 (PD-1) antibodies.</p><p><strong>Results: </strong>Long-time stimulated CD4 T-cells expressed high levels of Tim-3, but tended to decrease their PD-1 expression. Tim-3 expression was exclusively seen co-expressed with PD-1 by CD3, CD4, CD45RO positive cells in the inflamed RA joint. Addition of neutralizing Tim-3 antibodies increased the secretion of IFNγ and MCP-1, in SFMC cultures from RA. Whereas neutralizing anti-PD-1 antibodies showed a broader impact on cytokine production. Finally, we observed that soluble Tim-3 is increased in plasma and is associated with disease activity in early RA.</p><p><strong>Conclusion: </strong>Taken together, our findings indicate disease-suppressive functions of Tim-3 in RA.</p>","PeriodicalId":72163,"journal":{"name":"American journal of clinical and experimental immunology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301058/pdf/ajcei0011-0034.pdf","citationCount":"0","resultStr":"{\"title\":\"T-cell immunoglobulin and mucin domain 3 is upregulated in rheumatoid arthritis, but insufficient in controlling inflammation.\",\"authors\":\"Caecilie Skejoe,&nbsp;Aida S Hansen,&nbsp;Kristian Stengaard-Pedersen,&nbsp;Peter Junker,&nbsp;Kim Hoerslev-Pedersen,&nbsp;Merete L Hetland,&nbsp;Mikkel Oestergaard,&nbsp;Stinne Greisen,&nbsp;Malene Hvid,&nbsp;Mette Deleuran,&nbsp;Bent Deleuran\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Rheumatoid arthritis (RA) is a chronic autoimmune disease, that involves both pro- and anti-inflammatory mechanisms. The purpose of the present study is to investigate T-cell immunoglobulin and mucin domain 3 (Tim-3) in RA.</p><p><strong>Methods: </strong>Plasma levels of soluble (s) Tim-3 in early RA (n=98), were followed, to evaluate association with treatment and disease activity, acquired from a prospective collected biobank (clinicaltrials.gov (NCT00660647)). We also investigate the influence of Tim-3 on spontaneous cytokine production in synovial fluid mononuclear cells (SFMC) from RA patients after addition of neutralizing anti-Tim-3's antibodies, either alone or in combination with neutralizing anti-Programmed Cell death protein 1 (PD-1) antibodies.</p><p><strong>Results: </strong>Long-time stimulated CD4 T-cells expressed high levels of Tim-3, but tended to decrease their PD-1 expression. Tim-3 expression was exclusively seen co-expressed with PD-1 by CD3, CD4, CD45RO positive cells in the inflamed RA joint. Addition of neutralizing Tim-3 antibodies increased the secretion of IFNγ and MCP-1, in SFMC cultures from RA. Whereas neutralizing anti-PD-1 antibodies showed a broader impact on cytokine production. Finally, we observed that soluble Tim-3 is increased in plasma and is associated with disease activity in early RA.</p><p><strong>Conclusion: </strong>Taken together, our findings indicate disease-suppressive functions of Tim-3 in RA.</p>\",\"PeriodicalId\":72163,\"journal\":{\"name\":\"American journal of clinical and experimental immunology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2022-06-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301058/pdf/ajcei0011-0034.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American journal of clinical and experimental immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of clinical and experimental immunology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

目的:类风湿关节炎(RA)是一种慢性自身免疫性疾病,涉及促炎和抗炎机制。本研究的目的是探讨t细胞免疫球蛋白和粘蛋白结构域3 (Tim-3)在RA中的作用。方法:从前瞻性收集的生物库(clinicaltrials.gov (NCT00660647))获取早期RA (n=98)患者血浆可溶性Tim-3水平,以评估其与治疗和疾病活动度的关系。我们还研究了单独或联合中和抗程序性细胞死亡蛋白1 (PD-1)抗体后,Tim-3对RA患者滑液单核细胞(SFMC)自发细胞因子产生的影响。结果:长期刺激的CD4 t细胞Tim-3表达水平较高,而PD-1表达水平有降低的趋势。Tim-3仅在炎症关节炎关节的CD3、CD4、CD45RO阳性细胞中与PD-1共表达。在RA的SFMC培养物中,加入中和Tim-3抗体可增加IFNγ和MCP-1的分泌。而中和抗pd -1抗体对细胞因子产生更广泛的影响。最后,我们观察到可溶性Tim-3在血浆中增加,并且与早期RA的疾病活动性有关。结论:综上所述,我们的研究结果表明Tim-3在RA中具有疾病抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
T-cell immunoglobulin and mucin domain 3 is upregulated in rheumatoid arthritis, but insufficient in controlling inflammation.

Objectives: Rheumatoid arthritis (RA) is a chronic autoimmune disease, that involves both pro- and anti-inflammatory mechanisms. The purpose of the present study is to investigate T-cell immunoglobulin and mucin domain 3 (Tim-3) in RA.

Methods: Plasma levels of soluble (s) Tim-3 in early RA (n=98), were followed, to evaluate association with treatment and disease activity, acquired from a prospective collected biobank (clinicaltrials.gov (NCT00660647)). We also investigate the influence of Tim-3 on spontaneous cytokine production in synovial fluid mononuclear cells (SFMC) from RA patients after addition of neutralizing anti-Tim-3's antibodies, either alone or in combination with neutralizing anti-Programmed Cell death protein 1 (PD-1) antibodies.

Results: Long-time stimulated CD4 T-cells expressed high levels of Tim-3, but tended to decrease their PD-1 expression. Tim-3 expression was exclusively seen co-expressed with PD-1 by CD3, CD4, CD45RO positive cells in the inflamed RA joint. Addition of neutralizing Tim-3 antibodies increased the secretion of IFNγ and MCP-1, in SFMC cultures from RA. Whereas neutralizing anti-PD-1 antibodies showed a broader impact on cytokine production. Finally, we observed that soluble Tim-3 is increased in plasma and is associated with disease activity in early RA.

Conclusion: Taken together, our findings indicate disease-suppressive functions of Tim-3 in RA.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Advance in the mechanism and clinical research of myalgia in long COVID. Characterization of tumor suppressors and oncogenes evaluated from TCGA cancers. Clinical utility of multi-row spiral CT in diagnosing hepatic nodular lesions, gastric cancer, and Crohn's disease: a comprehensive meta-analysis. Effectiveness study of surface electromyography combined with spine 3D data system to evaluate scoliosis. The relationship between metabolites and gout: a Mendelian randomization study.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1