亚胺培南/西司他丁纳米脂质体对临床铜绿假单胞菌的抗菌膜增强和生长抑制作用

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2023-09-21 DOI:10.1007/s10856-023-06752-0
Faezeh Milani, Khosro Adibkia, Hamed Hamishehkar, Tooba Gholikhani, Farhad Bani, Morteza Milani
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引用次数: 0

摘要

许多感染与铜绿假单胞菌有关。由于毒力和抗生素耐药性,它是主要的医学问题之一。脂质体中的抗生素包封是控制由这种微生物引起的感染的良好策略。亚胺培南/西司他丁脂质体抗铜绿假单胞菌作用的体外评价。采用琼脂扩散法对分离株进行了耐药性鉴定。在使用薄层和乙醇注射技术制成纳米脂质体后,将抗生素放入纳米脂质体中。使用SEM和DLS来确定包封的药物形式和空的纳米脂质体的尺寸、形状和ζ电位。此外,利用FT-IR光谱研究了亚胺培南/西司他丁在纳米脂质体中的包封。在微生物测定实验中,测定了脂质体和游离药物形式的MIC、MBC和MBEC。纳米颗粒是球形的,直径从30到39 nm,并且薄层和乙醇注射程序中的EE%分别为97和98。亚胺培南/西司他丁纳米脂质体在3009处显示峰值 cm−1和1650 cm−1,证明了包封药物化学结构的热力学稳定性,并验证了抗生素在纳米脂质体中的包封。与游离药物形式相比,纳米脂质体在大多数分离物中具有较低的MIC和MBC值,并且具有更大的根除生物膜形成的能力。研究表明,这两种纳米脂质体制备技术在80%的分离株中更有效,其结果与许多其他研究的结果一致。总体而言,基于对其MIC和MBC的评估,我们证明纳米脂质体的抗菌活性高于游离药物形式。药物纳米脂质体技术为如何管理对抗生素具有耐药性的微生物感染提供了一个极好的未来前景。图形摘要
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Increased antibiofilm and growth inhibitory effect of Imipenem/Cilastatin nanoliposomes against clinical Pseudomonas aeruginosa isolates

Numerous infections are linked to Pseudomonas aeruginosa. It is one of the major medical concerns because of virulence and antibiotic resistance. Antibiotic encapsulation in liposomes is a good strategy for controlling infections caused by this microorganism. Evaluation of anti-Pseudomonas aeruginosa effect of liposomal form of Imipenem/Cilastatin in vitro condition. By using the disk agar diffusion technique, the isolates’ pattern of antibiotic resistance was identified. The antibiotic was placed into the nanoliposome after it had been made using the thin layer and ethanol injection techniques. SEM and DLS were used to determine the size, shape, and zeta potential of the encapsulated drug form and the empty nanoliposome. Additionally, Imipenem/Cilastatin encapsulation in nanoliposomes was studied using FT-IR spectroscopy. In the microbial assay experiments the MIC, MBC and MBEC of liposomal and free drug forms were determined. The nanoparticles were spherical, with a diameter ranging from 30 to 39 nm, and the EE% in the thin layer and ethanol injection procedures were 97 and 98, respectively. Imipenem/Cilastatin nanoliposomes showed peaks at 3009 cm−1 and 1650 cm−1, demonstrating the thermodynamic stability for the chemical structure of the drug enclosed and validating the encapsulation of antibiotic in the nanoliposomes. When compared to free drug forms, nanoliposomes had lower MIC and MBC values in the majority of the isolates and had a greater ability to eradicate the biofilm formation. It was shown that the two nanoliposome preparation techniques were more efficient in 80% of the isolates, which had outcomes that were consistent with those of numerous other investigations. Overall, we demonstrated that the antibacterial activity of nanoliposomes was higher than that of the free drug form based on the evaluation of their MIC and MBC. Pharmaceutical nanoliposome techniques provide an excellent future perspective on how to manage microbial infections that are resistant to antibiotics.

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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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